| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5B21 |
| Uniprot No | A6NL26 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MENSTEVTEFILLGLTDDPNLQIPLLLAFLFIYLITLLGNGGMMVIIHSDSHLHTPMYFF LSNLSLVDLGYSSAVAPKTVAALRSGDKAISYDGCAAQFFFFVGFATVECYLLASMAYDR HAAVCRPLHYTTTMTAGVCALLATGSYVSGFLNASIHAAGTFRLSFCGSNEINHFFCDIP PLLALSCSDTRISKLVVFVAGFNVFFTLLVILISYFFICITIQRMHSAEGQKKVFSTCAS HLTALSIFYGTIIFMYLQPNSSQSVDTDKIASVFYTVVIPMLNPLIYSLRNKEVKSALWK ILNKLYPQY |
| 分子量 | 34.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于嗅觉受体重组蛋白研究的常见主题构建的假设性参考文献示例，供参考(需确认实际文献存在性)：
1. **文献名称**：**"Functional expression of recombinant human olfactory receptor OR5B21 in HEK293 cells for ligand screening"**
**作者**：Smith A, et al.
**摘要**：本研究成功在HEK293细胞中表达OR5B21蛋白，通过钙离子荧光检测筛选出多种潜在气味分子配体，揭示了其感知特定酮类化合物的功能。
2. **文献名称**：**"Structural insights into OR5B21 through cryo-EM analysis of the recombinant protein complex"**
**作者**：Zhang L, et al.
**摘要**：利用昆虫细胞-杆状病毒系统重组表达OR5B21.结合冷冻电镜解析其三维结构，探讨了该受体与配体结合的关键结构域。
3. **文献名称**：**"OR5B21 as a novel biomarker: Recombinant protein production and validation in clinical samples"**
**作者**：Kim J, et al.
**摘要**：开发大肠杆菌重组OR5B21的高效表达与纯化方法，应用于抗体开发，并在特定癌症组织中发现其异常表达。
**注意**：以上文献为示例，实际研究中OR5B21可能研究较少。建议通过**PubMed**或**Google Scholar**以“OR5B21”、“olfactory receptor recombinant expression”为关键词检索最新文献。若需具体论文协助，请提供更多背景信息。
OR5B21 is a recombinant human olfactory receptor protein belonging to the G protein-coupled receptor (GPCR) superfamily. As part of the olfactory receptor (OR) family, it is encoded by the OR5B21 gene located on chromosome 11. Olfactory receptors typically detect odorant molecules in the nasal epithelium, but some, including OR5B21. are ectopically expressed in non-olfactory tissues, suggesting potential roles in cellular signaling beyond smell. Structurally, it features seven transmembrane domains and interacts with specific odorants or ligands, triggering intracellular cAMP-mediated signaling pathways via G protein coupling.
Recombinant OR5B21 is produced using heterologous expression systems (e.g., mammalian or insect cells) for functional studies, as native ORs are challenging to express in vitro. Its recombinant form enables research into ligand specificity, receptor activation mechanisms, and structure-function relationships. Emerging studies propose associations with physiological processes such as metabolic regulation and cell proliferation, with possible implications in diseases like cancer. However, its precise endogenous ligands and biological roles remain largely uncharacterized, making OR5B21 a subject of ongoing exploration in chemosensory biology and therapeutic target discovery.
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