| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5C1 |
| Uniprot No | Q8NGR4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-320 aa |
| 活性数据 | MNSENLTRAAVAPAEFVLLGITNRWDLRVALFLTCLPVYLVSLLGNMGMALLIRMDARLH TPMYFFLANLSLLDACYSSAIGPKMLVDLLLPRATIPYTACALQMFVFAGLADTECCLLA AMAYDRYVAIRNPLLYTTAMSQRLCLALLGASGLGGAVSAFVHTTLTFRLSFCRSRKINS FFCDIPPLLAISCSDTSLNELLLFAICGFIQTATVLAITVSYGFIAGAVIHMRSVEGSRR AASTGGSHLTAVAMMYGTLIFMYLRPSSSYALDTDKMASVFYTLVIPSLNPLIYSLRNKE VKEALRQTWSRFHCPGQGSQ |
| 分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5C1蛋白的参考文献示例（注：以下内容为模拟示例，实际文献需通过学术数据库查询）：
1. **文献名称**: "Heterologous Expression and Functional Characterization of Human Olfactory Receptor OR5C1 in HEK293 Cells"
**作者**: Smith A, et al.
**摘要**: 本研究成功在HEK293细胞中表达重组OR5C1蛋白，并通过钙流实验证实其对特定醛类化合物（如辛醛）的响应，揭示了其在嗅觉信号传导中的作用。
2. **文献名称**: "Structural Insights into OR5C1: Cryo-EM Analysis of a Human Olfactory Receptor"
**作者**: Lee B, et al.
**摘要**: 通过冷冻电镜解析了重组OR5C1蛋白的三维结构，揭示了其配体结合口袋的构象，为嗅觉受体家族的结构-功能关系研究提供了新线索。
3. **文献名称**: "Optimized Bacterial Expression and Purification of OR5C1 for High-Throughput Ligand Screening"
**作者**: Chen L, et al.
**摘要**: 开发了一种基于大肠杆菌的重组OR5C1表达和纯化方案，结合SPR技术筛选潜在配体，拓展了其在化学生物学中的应用。
4. **文献名称**: "OR5C1 Expression in Non-Olfactory Tissues: Implications in Cancer Cell Migration"
**作者**: Wang Y, et al.
**摘要**: 报道OR5C1在乳腺癌细胞中异常表达，重组蛋白功能实验表明其可能通过调控细胞迁移相关通路参与肿瘤转移。
**注意事项**：以上为假设性案例，真实文献需通过PubMed、Web of Science等平台检索关键词（如"OR5C1 recombinant"、"olfactory receptor 5C1"）。若研究较少，可扩展至嗅觉受体家族重组表达技术或OR5C1相关通路的研究。
**Background of Recombinant Human OR5C1 Protein**
The olfactory receptor family 5 subfamily C member 1 (OR5C1) is a G protein-coupled receptor (GPCR) primarily associated with olfactory sensory neurons, where it plays a role in detecting odorants. While traditionally linked to smell perception, recent studies highlight its ectopic expression in non-olfactory tissues, suggesting broader physiological or pathological roles, such as in prostate cancer, metabolic regulation, and cellular signaling. OR5C1's specific ligands and downstream pathways remain partially characterized, though structural modeling indicates conserved GPCR features, including seven transmembrane domains and ligand-binding pockets.
Recombinant OR5C1 protein is generated using heterologous expression systems (e.g., mammalian cells, bacteria, or insect cells) to enable functional and structural studies. Its production facilitates investigations into receptor-ligand interactions, activation mechanisms, and potential therapeutic applications. Challenges include low natural abundance, membrane protein instability, and difficulties in purification. Recent advances in cryo-EM and computational biology have aided in resolving its conformational dynamics.
Emerging research implicates OR5C1 in disease contexts, such as cancer cell proliferation and obesity-related pathways, making it a target for drug discovery. Studies also explore its role in chemotaxis and intercellular communication. However, comprehensive data on its physiological ligands and tissue-specific functions remain limited, driving ongoing research to decode its biological significance beyond olfaction.
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