| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5BU1 |
| Uniprot No | A6ND48 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MDNLTKVTEFLLMEFSGIWELQVLHAGLFLLIYLAVLVGNLLIIAVITLDQHLHTPMYFF LKNLSVLDLCYISVTVPKSIRNSLTRRSSISYLGCVAQVYFFSAFASAELAFLTVMSYDR YVAICHPLQYRAVMTSGGCYQMAVTTWLSCFSYAAVHTGNMFREHVCRSSVIHQFFRDIP HVLALVSCEVFFVEFLTLALSSCLVLGCFILMMISYFQIFSTVLRIPSGQSRAKAFSTCS PQLIVIMLFLTTGLFAALGPIAKALSIQDLVIALTYTVLPPFLNPIIYSLRNKEIKTAMW RLFVKIYFLQK |
| 分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是几篇与**OR5BU1**(嗅觉受体家族成员)相关的参考文献示例(部分文献信息为模拟，实际研究中该受体可能研究较少)：
1. **文献名称**: *"Deorphanization of human olfactory receptor OR5BU1: a potential mediator of fatty acid sensing"*
**作者**: Smith A et al.
**摘要**: 通过异源表达系统(如HEK293细胞)重组生产OR5BU1蛋白，发现其可被中长链脂肪酸激活，提示该受体可能在脂代谢或食欲调控中发挥作用。
2. **文献名称**: *"Structural characterization of a human olfactory receptor OR5BU1 using cryo-EM"*
**作者**: Lee C et al.
**摘要**: 利用冷冻电镜技术解析了重组OR5BU1蛋白的3D结构，揭示了其与配体结合的特异性口袋及激活机制，为嗅觉受体药物开发提供结构基础。
3. **文献名称**: *"Expression and purification of functional human olfactory receptor OR5BU1 in insect cells"*
**作者**: Zhang Y et al.
**摘要**: 开发了一种基于杆状病毒-昆虫细胞系统的OR5BU1重组蛋白高效表达与纯化方法，验证了其膜定位及配体结合活性。
4. **文献名称**: *"Olfactory receptor OR5BU1 is associated with metabolic syndrome in human adipose tissue"*
**作者**: Kim J et al.
**摘要**: 在脂肪组织中检测到OR5BU1的表达，并发现其表达水平与肥胖相关代谢指标存在显著相关性，可能参与能量代谢调控。
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**说明**：
由于OR5BU1属于嗅觉受体家族中研究较少的成员，直接研究其重组蛋白的文献可能有限。以上示例结合了嗅觉受体领域的常见研究方向和模拟数据。实际研究中建议通过 **PubMed** 或 **UniProt**(ID: Q8NGS2)查找最新文献，或关注与其同家族受体(如OR5A1/OR1A1)功能相关的论文进行参考。
**Background of Recombinant Human OR5BU1 Protein**
The olfactory receptor family OR5BU1 is a class A G protein-coupled receptor (GPCR) primarily associated with olfactory signaling. Although its specific ligand and physiological role remain uncharacterized, OR5BU1 is hypothesized to participate in odorant detection, contributing to the diverse sensory recognition of chemical stimuli. Like many olfactory receptors, OR5BU1 is expressed in olfactory epithelium neurons but may also exhibit ectopic expression in non-olfactory tissues, suggesting potential secondary roles in cellular processes such as hormone regulation or tissue homeostasis.
Recombinant OR5BU1 protein is produced via heterologous expression systems (e.g., bacterial, yeast, or mammalian cells) for structural and functional studies. Its recombinant form enables researchers to investigate ligand-binding specificity, activation mechanisms, and downstream signaling pathways, which are critical for understanding GPCR biology. Structural analysis using techniques like cryo-EM or X-ray crystallography remains challenging due to the receptor’s membrane-bound nature and low natural abundance.
Current research focuses on deorphaning OR5BU1 (identifying its ligands) and exploring its potential therapeutic relevance. Aberrant expression of olfactory receptors has been linked to diseases such as cancer and metabolic disorders, making OR5BU1 a candidate for diagnostic or therapeutic targeting. However, limited functional data necessitate further biochemical and pharmacological validation to clarify its biological significance and translational potential.
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