| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5H1 |
| Uniprot No | A6NKK0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-313 aa |
| 活性数据 | MEEENATLLTEFVLTGFLYQPQWKIPLFLAFLVIYLITIMGNLGLIAVIWKDPHLHIPMY LLLGNLAFVDAWISSTVTPKMLNNFLAKSKMISLSECKIQFFSFAISVTTECFLLATMAY DRYVAICKPLLYPAIMTNGLCIRLLILSYVGGILHALIHEGFLFRLTFCNSNIVHHIYCD TIPLSKISCTDSSINFLMVFIFSGSIQVFSIVTILVSYTFVLFAILKKKSDKGVRKAFST CGAHLFSVSLYYGPLLFIYVGPASPQADDQDMVEPLFYTVIIPLLNPIIYSLRNKQVTVS FTKMLKKHVKVSY |
| 分子量 | 35.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5H1蛋白的3篇参考文献示例（基于近年研究推测，具体文献需核实）：
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1. **文献名称**: "Expression and Ligand Screening of Recombinant Olfactory Receptor OR5H1 in HEK293 Cells"
**作者**: Smith, J. et al. (2019)
**摘要**: 本研究在HEK293细胞中成功表达了重组人OR5H1蛋白，并通过荧光钙离子成像技术筛选了潜在配体，发现其可被特定类异戊二烯化合物激活，提示其在非嗅觉组织中的生理功能。
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2. **文献名称**: "Structural Insights into OR5H1 via Cryo-EM Reveal Odorant Binding Mechanisms"
**作者**: Lee, H. & Kim, S. (2021)
**摘要**: 利用冷冻电镜首次解析了重组OR5H1蛋白的三维结构，揭示了其与内源性配体（如雄烯酮衍生物）的结合位点，为嗅觉受体家族的结构-功能关系提供了新依据。
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3. **文献名称**: "OR5H1 Promotes Tumor Angiogenesis in Prostate Cancer via Recombinant Protein-based Assays"
**作者**: Zhang, Y. et al. (2020)
**摘要**: 通过重组OR5H1蛋白的体外实验，发现其与前列腺癌细胞分泌的VEGF存在互作，可能通过激活MAPK通路促进血管生成，为癌症治疗提供了潜在靶点。
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**备注**：上述文献为模拟示例，实际研究中OR5H1的相关报道可能有限。建议通过数据库（如PubMed）以关键词 **"OR5H1 recombinant"** 或 **"OR5H1 protein function"** 进一步检索最新文献。
**Background of Recombinant Human OR5H1 Protein**
The recombinant human OR5H1 protein is a chemically synthesized or expressed form of the olfactory receptor family 5 subfamily H member 1 (OR5H1), a class A G protein-coupled receptor (GPCR). OR5H1 is part of the olfactory receptor (OR) superfamily, which mediates odorant detection and signal transduction in the olfactory epithelium. These receptors are characterized by seven transmembrane domains and play critical roles in recognizing volatile odor molecules, initiating neural responses that contribute to the sense of smell. Although the specific ligands and physiological roles of OR5H1 remain largely uncharacterized, emerging evidence suggests its potential involvement in non-olfactory tissues, such as the reproductive system or brain, hinting at broader biological functions.
Recombinant OR5H1 is typically produced using heterologous expression systems (e.g., mammalian cells, insect cells, or *E. coli*) to enable structural and functional studies. Its production allows researchers to explore receptor-ligand interactions, signaling pathways, and potential therapeutic applications. Challenges in OR5H1 research include low natural expression levels and difficulties in solubilizing membrane-bound receptors. However, advances in protein engineering and cryo-EM have facilitated structural insights. OR5H1 is of interest in deciphering olfactory coding mechanisms, understanding ectopic receptor functions, and developing biosensors or targeted therapies for diseases linked to olfactory or GPCR dysregulation. Current studies focus on deorphanizing the receptor and validating its role in cellular processes beyond smell.
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