| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5K1 |
| Uniprot No | Q8NHB7 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-308 aa |
| 活性数据 | MAEENHTMKNEFILTGFTDHPELKTLLFVVFFAIYLITVVGNISLVALIFTHRRLHTPMY IFLGNLALVDSCCACAITPKMLENFFSENKRISLYECAVQFYFLCTVETADCFLLAAMAY DRYVAICNPLQYHIMMSKKLCIQMTTGAFIAGNLHSMIHVGLVFRLVFCGSNHINHFYCD ILPLYRLSCVDPYINELVLFIFSGSVQVFTIGSVLISYLYILLTIFKMKSKEGRAKAFST CASHFLSVSLFYGSLFFMYVRPNLLEEGDKDIPAAILFTIVVPLLNPFIYSLRNREVISV LRKILMKK |
| 分子量 | 35.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5K1蛋白的示例参考文献（文献信息为虚拟示例，实际需通过学术数据库验证）：
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1. **文献名称**：*Functional characterization of recombinant human olfactory receptor OR5K1 expressed in mammalian cells*
**作者**：Smith A, et al.
**摘要**：本研究通过哺乳动物细胞表达系统成功制备了重组OR5K1蛋白，并验证了其与特定挥发性有机分子（如2-壬酮）的结合活性，揭示了该受体在嗅觉信号传导中的潜在作用。
2. **文献名称**：*Structural insights into the ligand-binding specificity of OR5K1 using purified recombinant protein*
**作者**：Zhang L, et al.
**摘要**：利用重组OR5K1蛋白的纯化样品，结合冷冻电镜技术解析了其三维结构，发现了其配体结合口袋的关键氨基酸残基，为嗅觉受体家族的功能多样性提供结构依据。
3. **文献名称**：*Development of a high-throughput screening assay for OR5K1 ligands based on recombinant receptor expression*
**作者**：Johnson R, et al.
**摘要**：通过在HEK293细胞中稳定表达重组OR5K1，建立了基于钙离子荧光检测的配体高通量筛选平台，鉴定出多种新型激动剂和拮抗剂，推动嗅觉受体药物开发研究。
4. **文献名称**：*Comparative analysis of odorant receptor OR5K1 expression and function across species*
**作者**：Chen H, et al.
**摘要**：研究比较了人源和鼠源重组OR5K1蛋白的配体选择性和信号通路差异，表明物种特异性可能影响嗅觉感知机制。
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**注意**：以上为示例文献，实际研究需通过 **PubMed** 或 **Google Scholar** 检索真实文献（关键词：OR5K1, recombinant, olfactory receptor）。如需具体文献协助，请提供更详细的研究方向。
**Background of Recombinant Human OR5K1 Protein**
OR5K1, a member of the olfactory receptor (OR) family, is a G protein-coupled receptor (GPCR) primarily associated with olfactory sensory perception. These receptors detect odorant molecules, initiating signal transduction cascades that translate chemical cues into neural signals. OR5K1 is encoded by the *OR5K1* gene located on human chromosome 3. While its specific ligands and physiological roles remain under investigation, OR5K1 is hypothesized to participate in recognizing distinct odorants, contributing to the complexity of human olfaction.
Recombinant OR5K1 protein is produced via heterologous expression systems, such as mammalian cells (e.g., HEK293) or insect cell-baculovirus systems, to enable structural and functional studies. Its production often involves tagging (e.g., FLAG, His-tag) to facilitate purification and detection. Challenges in OR5K1 expression include low yields and membrane localization due to its hydrophobic nature, typical of GPCRs.
Research on recombinant OR5K1 aims to unravel its ligand specificity, activation mechanisms, and potential roles beyond olfaction, such as in neuroprotection or metabolic regulation. It also serves as a model for studying GPCR signaling and drug discovery, particularly for disorders linked to olfactory dysfunction. Despite progress, the lack of resolved 3D structures and limited ligand data highlight the need for further exploration of OR5K1’s molecular and therapeutic relevance.
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