| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR5H6 |
| Uniprot No | Q8NGV6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-325 aa |
| 活性数据 | MFLYLCFIFQRTCSEEMEEENATLLTEFVLTGFLHQPDCKIPLFLAFLVIYLITIMGNLG LIVLIWKDPHLHIPMYLFLGSLAFVDASLSSTVTPKMLINFLAKSKMISLSECMVQFFSL VTTVTTECFLLATMAYDRYVAICKALLYPVIMTNELCIQLLVLSFIGGLLHALIHEAFSF RLTFCNSNIIQHFYCDIIPLLKISCTDSSINFLMVFIFAGSVQVFTIGTILISYTIILFT ILEKKSIKGIRKAVSTCGAHLLSVSLYYGPLTFKYLGSASPQADDQDMMESLFYTVIVPL LNPMIYSLRNKQVIASFTKMFKSNV |
| 分子量 | 36.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR5H6蛋白的参考文献概述（注：OR5H6研究较为局限，以下示例基于文献结构模拟，建议通过学术数据库核实具体内容）：
1. **《Functional Characterization of Recombinant Human Olfactory Receptor OR5H6 through Heterologous Expression》**
*Authors: Smith A, et al.*
摘要：本研究在HEK293细胞中成功表达重组OR5H6蛋白，利用钙成像技术筛选其潜在气味配体，发现对植物挥发性化合物（如β-ionone）有特异性响应，揭示了其在气味感知中的作用机制。
2. **《Expression and Purification of Human Olfactory Receptor OR5H6 in a Baculovirus System》**
*Authors: Lee J, et al.*
摘要：通过杆状病毒-昆虫细胞系统表达OR5H6重组蛋白，优化了去污剂提取和亲和层析纯化方法，为后续结构解析和功能研究提供了技术基础。
3. **《OR5H6: A Novel Biomarker in Endometrial Cancer Identified via Recombinant Protein Analysis》**
*Authors: Garcia R, et al.*
摘要：通过重组OR5H6蛋白的体外实验，发现其在子宫内膜癌组织中异常高表达，可能参与肿瘤细胞迁移，提示其作为诊断或治疗靶点的潜力。
4. **《GPCR Signaling Modulation of Recombinant OR5H6 in Cellular Models》**
*Authors: Kumar P, et al.*
摘要：结合重组OR5H6蛋白的细胞模型，揭示了其通过cAMP/PKA通路调控下游信号，并探讨了跨膜结构域关键氨基酸位点对受体激活的影响。
**备注**：上述文献为示例性质，实际研究可能需查询**PubMed/Google Scholar**等数据库获取，关键词推荐："OR5H6 recombinant expression"、"OR5H6 functional analysis"。
OR5H6 is a member of the olfactory receptor (OR) family, a class of G protein-coupled receptors (GPCRs) predominantly expressed in olfactory sensory neurons. These receptors detect odorant molecules, initiating signal transduction that contributes to the sense of smell. The OR5H6 gene, located on human chromosome 11, encodes a protein characterized by seven transmembrane domains and ligand-binding specificity, though its exact endogenous ligands remain under investigation. As a "class II" OR, it is evolutionarily tuned to recognize a subset of odorants, potentially including volatile organic compounds.
Recombinant OR5H6 protein is produced via heterologous expression systems (e.g., HEK293 cells or insect cell lines) to study its structural and functional properties. Its recombinant form enables research into odorant-receptor interaction mechanisms, receptor activation pathways, and structure-function relationships. Notably, OR5H6 has been implicated in non-olfactory contexts, with emerging studies suggesting ectopic expression in tissues like the prostate and roles in cellular processes beyond chemosensation.
Pharmaceutically, OR5H6 serves as a model for GPCR-targeted drug design. Research also explores its potential involvement in diseases; for example, dysregulation of ORs is linked to neurodegenerative disorders. However, challenges persist in deorphaning OR5H6 (identifying its natural ligands) and elucidating its full physiological scope. Current work leverages cryo-EM and computational modeling to advance therapeutic applications and decode its biological significance.
×
