| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR8B4 |
| Uniprot No | Q96RC9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-309 aa |
| 活性数据 | MTLRNSSSVTEFILVGLSEQPELQLPLFLLFLGIYVFTVVGNLGLITLIGINPSLHTPMY FFLFNLSFIDLCYSCVFTPKMLNDFVSESIISYVGCMTQLFFFCFFVNSECYVLVSMAYD RYVAICNPLLYMVTMSPRVCFLLMFGSYVVGFAGAMAHTGSMLRLTFCDSNVIDHYLCDV LPLLQLSCTSTHVSELVFFIVVGVITMLSSISIVISYALILSNILCIPSAEGRSKAFSTW GSHIIAVALFFGSGTFTYLTTSFPGSMNHGRFASVFYTNVVPMLNPSIYSLRNKDDKLAL GKTLKRVLF |
| 分子量 | 34.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于**重组人OR8B4蛋白**的示例参考文献（注：部分内容为示例性概括，实际文献可能需要根据具体研究方向检索）:
1. **《Functional expression of human olfactory receptor OR8B4 in HEK293 cells》**
- 作者：Smith A, et al.
- 摘要：报道了利用HEK293细胞成功重组表达OR8B4蛋白，并通过cAMP信号通路分析证实其能够响应特定挥发性配体，为研究OR8B4的嗅觉功能提供了模型。
2. **《OR8B4 as a novel biomarker in colorectal cancer: Expression and ligand interaction studies》**
- 作者：Zhang L, et al.
- 摘要：通过免疫组化与重组蛋白技术，发现OR8B4在结直肠癌组织中异常高表达，并鉴定了其与某种脂肪酸衍生物的相互作用，提示其可能参与肿瘤代谢调控。
3. **《Structural insights into the ligand-binding specificity of human OR8B4 olfactory receptor》**
- 作者：Jones R, et al.
- 摘要：基于重组OR8B4蛋白的晶体结构解析，揭示了其配体结合口袋的独特氨基酸残基组合，解释了其对中链醛类分子的选择性识别机制。
4. **《A high-throughput screening platform for deorphanizing human olfactory receptors: Case study on OR8B4》**
- 作者：Kim H, et al.
- 摘要：开发了一种基于荧光报告基因的重组OR8B4功能筛选平台，筛选了超过1000种气味分子，发现辛醛为其潜在高亲和力配体。
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**注意**：上述文献为基于OR8B4可能研究方向的模拟示例，实际文献需通过PubMed、Web of Science等数据库检索确认。建议使用关键词“OR8B4”、“olfactory receptor 8B4”、“recombinant OR8B4”查找最新研究。
**Background of Recombinant Human OR8B4 Protein**
The olfactory receptor family OR8B4, part of the G protein-coupled receptor (GPCR) superfamily, is primarily associated with the detection of odorants in the olfactory system. These receptors are expressed in olfactory sensory neurons within the nasal epithelium, where they bind to specific volatile chemical compounds, triggering signal transduction pathways that contribute to the sense of smell. OR8B4, encoded by the *OR8B4* gene, is one of hundreds of olfactory receptors (ORs) in humans, which collectively enable the recognition of a vast array of odor molecules through combinatorial activation patterns.
Recombinant OR8B4 protein refers to the engineered form of this receptor produced in heterologous expression systems (e.g., mammalian, insect, or bacterial cells). Its production enables structural and functional studies to elucidate its ligand specificity, activation mechanisms, and role in olfactory signaling. Recent research also explores potential "ectopic" expression of ORs, including OR8B4, in non-olfactory tissues, suggesting roles in cellular processes beyond smell, such as metabolic regulation or disease pathways.
Studying recombinant OR8B4 aids in understanding olfactory receptor biology, drug discovery (targeting ORs for therapeutic modulation), and biosensor development. Challenges include maintaining receptor stability and proper folding *in vitro*, which often requires optimized expression systems and lipid bilayer mimetics. Advances in cryo-EM and GPCR pharmacology continue to enhance insights into OR8B4's 3D structure and ligand-binding dynamics.
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