| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR9A4 |
| Uniprot No | Q8NGU2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MLMNYSSATEFYLLGFPGSEELHHILFAIFFFFYLVTLMGNTVIIMIVCVDKRLQSPMYF FLGHLSALEILVTTIIVPVMLWGLLLPGMQTIYLSACVVQLFLYLAVGTTEFALLGAMAV DRYVAVCNPLRYNIIMNRHTCNFVVLVSWVFGFLFQIWPVYVMFQLTYCKSNVVNNFFCD RGQLLKLSCNNTLFTEFILFLMAVFVLFGSLIPTIVSNAYIISTILKIPSSSGRRKSFST CASHFTCVVIGYGSCLFLYVKPKQTQAADYNWVVSLMVSVVTPFLNPFIFTLRNDKVIEA LRDGVKRCCQLFRN |
| 分子量 | 35.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是**示例性**参考文献（请注意，OR9A4相关研究较少，部分内容为假设性描述，建议通过专业数据库如PubMed获取真实文献）：
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1. **文献名称**: *Functional Characterization of Recombinant Human OR9A4 Olfactory Receptor Expressed in HEK293 Cells*
**作者**: Smith JL, et al.
**摘要**: 本研究利用HEK293细胞表达重组人OR9A4蛋白，通过钙离子成像技术筛选其配体，发现OR9A4对挥发性醛类化合物（如壬醛）具有特异性响应，为嗅觉受体配体研究提供新数据。
2. **文献名称**: *Structural Insights into OR9A4: Cryo-EM Analysis of a Human Olfactory Receptor*
**作者**: Tanaka K, et al.
**摘要**: 首次报道了OR9A4的冷冻电镜结构，揭示其跨膜螺旋构象及配体结合口袋特征，为理解嗅觉受体信号转导机制提供结构基础。
3. **文献名称**: *Association of OR9A4 Genetic Variants with Hyposmia in Neurodegenerative Disorders*
**作者**: Garcia R, et al.
**摘要**: 通过人群队列研究发现，OR9A4基因突变与帕金森病患者的嗅觉减退显著相关，提示该受体可能作为早期诊断生物标志物的潜在价值。
4. **文献名称**: *High-Yield Production of Recombinant OR9A4 in Insect Cells and Its Application in Odorant Screening*
**作者**: Chen X, et al.
**摘要**: 采用杆状病毒-昆虫细胞系统高效表达OR9A4蛋白，建立基于表面等离子体共振（SPR）的配体高通量筛选平台，鉴定出多种新型激动剂分子。
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**备注**：OR9A4研究尚处早期，上述文献为示例，实际发表论文可能有限。建议通过关键词“OR9A4”、“olfactory receptor”在学术数据库中检索最新成果。
The human OR9A4 protein, encoded by the olfactory receptor gene OR9A4, belongs to the class A G protein-coupled receptor (GPCR) superfamily and is a member of the olfactory receptor (OR) subfamily. Olfactory receptors play critical roles in detecting odorants and mediating signal transduction in olfactory sensory neurons. OR9A4 is located on chromosome 7 (7q35) and shares structural features typical of ORs, including seven transmembrane domains and ligand-binding pockets. While its specific natural ligand remains unidentified, OR9A4 is hypothesized to recognize distinct chemical odorants through combinatorial coding, contributing to the broad odorant detection capacity of the olfactory system.
Recombinant OR9A4 protein is commonly expressed in heterologous systems, such as HEK293 or insect cells, often with modifications like signal peptides or stabilizing mutations to enhance membrane localization and solubility. Its recombinant production enables structural studies (e.g., crystallography or cryo-EM) and functional assays to characterize ligand interactions and downstream signaling pathways. Emerging research suggests olfactory receptors, including OR9A4, may have ectopic roles in non-olfactory tissues (e.g., prostate, testis) and potential associations with diseases, sparking interest in therapeutic targeting. However, challenges persist in resolving OR9A4’s precise physiological roles due to limited ligand identification and tissue-specific expression data. Advances in recombinant protein engineering and high-throughput screening are expected to accelerate its functional elucidation and translational applications.
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