| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR8G1 |
| Uniprot No | Q15617 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MSGENNSSVTEFILAGLSEQPELQLPLFLLFLGIYVVTVVGNLGMTTLIWLSSHLHTPMY YFLSSLSFIDFCHSTVITPKMLVNFVTEKNIISYPECMTQLYFFLVFAIAECHMLAAMAY DRYMAICSPLLYSVIISNKACFSLILGVYIIGLVCASVHTGCMFRVQFCKFDLINHYFCD LLPLLKLSCSSIYVNKLLILCVGAFNILVPSLTILCSYIFIIASILHIRSTEGRSKAFST CSSHMLAVVIFFGSAAFMYLQPSSISSMDQGKVSSVFYTIIVPMLNPLIYSLRNKDVHVS LKKMLQRRTLL |
| 分子量 | 34.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR8G1蛋白的示例参考文献（基于相关领域研究的推断性示例，非真实文献）：
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1. **《Functional expression and ligand identification of the human olfactory receptor OR8G1 in heterologous cells》**
- 作者：Smith J. et al.
- 摘要：本研究通过将OR8G1基因克隆至HEK293细胞系中成功表达重组OR8G1蛋白，并利用钙离子成像技术筛选其潜在配体，发现该受体对特定挥发性醛类化合物具有响应性。
2. **《Structural characterization of OR8G1 using cryo-electron microscopy reveals odorant binding mechanisms》**
- 作者：Wang L. & Zhang H.
- 摘要：通过冷冻电镜解析了重组OR8G1蛋白的三维结构，揭示了其与气味分子结合的关键氨基酸残基及跨膜结构域的构象变化，为嗅觉受体活化机制提供结构基础。
3. **《OR8G1 signaling pathway analysis in non-olfactory tissues: Implications for prostate cancer progression》**
- 作者：Kim S. et al.
- 摘要：研究重组OR8G1蛋白在前列腺癌细胞中的表达，发现其通过激活cAMP信号通路调控细胞迁移，提示OR8G1可能在肿瘤转移中发挥作用。
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**注**：以上为模拟示例，实际文献需通过PubMed、Google Scholar等平台检索关键词（如“OR8G1 recombinant protein”“OR8G1 olfactory receptor”）获取。真实研究多聚焦于嗅觉受体的配体鉴定、结构解析或非嗅觉功能。
**Background of Recombinant Human OR8G1 Protein**
The human OR8G1 protein, encoded by the *OR8G1* gene, is a member of the olfactory receptor (OR) family, which belongs to the G protein-coupled receptor (GPCR) superfamily. Olfactory receptors are crucial for detecting odorant molecules, enabling the sense of smell. OR8G1 is classified under the OR8G subfamily and is located on chromosome 11. Structurally, it features seven transmembrane domains, characteristic of GPCRs, and is primarily expressed in olfactory sensory neurons.
Recombinant OR8G1 protein is engineered through heterologous expression systems (e.g., mammalian, insect, or bacterial cells) to study its ligand specificity, signaling mechanisms, and structural properties. Producing functional OR8G1 recombinantly is challenging due to the complexity of membrane protein folding and stabilization. Researchers often employ solubilization techniques, affinity tags, or lipid-based systems to enhance yield and activity.
Although OR8G1's precise natural ligands remain under investigation, recombinant versions facilitate high-throughput screening to identify potential odorant activators or inhibitors. This protein also serves as a model to explore olfactory coding mechanisms, receptor-ligand dynamics, and evolutionary conservation within the OR family. Additionally, it has implications in studying non-olfactory roles, such as potential functions in other tissues or diseases. Advances in OR8G1 research contribute to broader applications in biotechnology, including biosensor development and targeted therapies. Current studies focus on resolving its 3D structure and elucidating its role in human chemosensation and beyond.
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