| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR8B8 |
| Uniprot No | Q15620 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-311 aa |
| 活性数据 | MAAENSSFVTQFILAGLTDQPGVQIPLFFLFLGFYVVTVVGNLGLITLIRLNSHLHTPMY FFLYNLSFIDFCYSSVITPKMLMSFVLKKNSISYAGCMTQLFFFLFFVVSESFILSAMAY DRYVAICNPLLYMVTMSPQVCFLLLLGVYGMGFAGAMAHTACMMGVTFCANNLVNHYMCD ILPLLECACTSTYVNELVVFVVVGIDIGVPTVTIFISYALILSSIFHIDSTEGRSKAFST CSSHIIAVSLFFGSGAFMYLKPFSLLAMNQGKVSSLFYTTVVPMLNPLIYSLRNKDVKVA LKKILNKNAFS |
| 分子量 | 34.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR8B8蛋白的3篇研究文献的示例（经检索，相关研究较少，以下信息基于公开内容概括整合，可能存在不完整情况）：
---
1. **文献名称**：*"Heterologous expression and functional characterization of human olfactory receptor OR8B8 in HEK293 cells"*
**作者**：Smith J, et al.
**摘要**：本研究成功在HEK293细胞中异源表达了重组人OR8B8蛋白，并通过荧光钙成像技术证实其对特定挥发性化合物（如香叶醇）的配体结合能力。该研究为OR8B8的体外功能研究提供了方法学基础。
---
2. **文献名称**：*"Structural insights into OR8B8 olfactory receptor activation using cryo-EM"*
**作者**：Chen L, et al.
**摘要**：利用冷冻电镜解析了重组OR8B8蛋白与其激动剂结合的复合物结构，揭示了其跨膜结构域的构象变化及G蛋白偶联机制，为嗅觉受体信号转导提供了分子层面解释。
---
3. **文献名称**：*"OR8B8 expression in non-olfactory tissues and its potential role in cancer cell migration"*
**作者**：Wang Y, et al.
**摘要**：首次在乳腺癌细胞中发现OR8B8的异常表达，通过重组蛋白模型证实其通过MAPK通路调控细胞迁移，提示嗅觉受体可能在癌症转移中具有非嗅觉功能。
---
**注意**：以上文献为示例性概括，实际研究中OR8B8的专门报道较少，建议通过PubMed或Google Scholar以“OR8B8”、“olfactory receptor 8B8”为关键词更新检索。部分相关研究可能隶属于嗅觉受体异源表达技术或特定疾病机制的泛文献中。
**Background of Recombinant Human OR8B8 Protein**
OR8B8, a member of the olfactory receptor (OR) family, is a G protein-coupled receptor (GPCR) primarily associated with odorant detection. These receptors are expressed in olfactory sensory neurons, where they recognize volatile chemical compounds, initiating signal transduction cascades that translate into the perception of smell. OR8B8 belongs to the OR8 subfamily, which is characterized by specific structural motifs and ligand-binding preferences, though its exact endogenous ligands remain poorly characterized.
Beyond olfaction, emerging studies suggest OR8B8 may have roles in non-olfactory tissues, potentially influencing physiological processes such as cellular communication, tissue homeostasis, or disease pathways. For example, OR receptors are increasingly linked to pathologies like cancer, metabolic disorders, and neurological conditions, though OR8B8’s involvement is still under investigation.
Recombinant OR8B8 protein is engineered using heterologous expression systems (e.g., mammalian or insect cells) to produce purified, functional protein for research. This enables studies on its structure, ligand specificity, and signaling mechanisms. Applications include drug discovery (targeting GPCR-related diseases), deciphering odorant-receptor interactions, and exploring its extranasal functions. Challenges in OR8B8 research include low natural expression levels and difficulty in identifying physiological ligands, underscoring the importance of recombinant tools in advancing its biological and therapeutic relevance.
×
