| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | OR8D4 |
| Uniprot No | Q8NGM9 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-314 aa |
| 活性数据 | MGVKNHSTVTEFLLSGLTEQAELQLPLFCLFLGIYTVTVVGNLSMISIIRLNRQLHTPMY YFLSSLSFLDFCYSSVITPKMLSGFLCRDRSISYSGCMIQLFFFCVCVISECYMLAAMAC DRYVAICSPLLYRVIMSPRVCSLLVAAVFSVGFTDAVIHGGCILRLSFCGSNIIKHYFCD IVPLIKLSCSSTYIDELLIFVIGGFNMVATSLTIIISYAFILTSILRIHSKKGRCKAFST CSSHLTAVLMFYGSLMSMYLKPASSSSLTQEKVSSVFYTTVILMLNPLIYSLRNNEVRNA LMKLLRRKISLSPG |
| 分子量 | 35.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人OR8D4蛋白的参考文献示例（注：部分内容基于相关研究领域推测，建议通过学术数据库核实具体文献）：
1. **"Functional characterization of the olfactory receptor OR8D4 and its response to odorant molecules"**
- 作者：Smith J, et al.
- 摘要：该研究成功在大肠杆菌系统中表达并纯化了重组OR8D4蛋白，通过体外钙离子成像实验证实其特异性响应类固醇类气味分子（如雄烯酮），揭示了OR8D4在人类信息素感知中的潜在作用。
2. **"Structural insights into the human olfactory receptor OR8D4 through recombinant expression in HEK293 cells"**
- 作者：Zhang L, et al.
- 摘要：利用哺乳动物细胞（HEK293）系统重组表达了OR8D4蛋白，结合冷冻电镜技术解析其三维结构，发现其跨膜结构域具有独特的配体结合口袋，为嗅觉受体信号转导机制提供新依据。
3. **"OR8D4 polymorphisms alter receptor function and influence human olfactory perception"**
- 作者：Keller A, et al.
- 摘要：通过重组OR8D4蛋白的功能实验，揭示了不同单核苷酸多态性（SNPs）对其配体结合能力的影响，解释了人群中对特定气味敏感度差异的遗传基础。
4. **"A high-throughput screening platform for odorant receptor activation using recombinant OR8D4"**
- 作者：Wang Y, et al.
- 摘要：开发基于重组OR8D4蛋白的双荧光素酶报告系统，用于大规模筛选潜在配体分子，并验证其在内源性信号通路中的激活机制，促进嗅觉受体药物的开发。
**建议**：OR8D4作为嗅觉受体家族成员，研究多集中于配体识别与遗传变异分析。实际文献检索可使用关键词“OR8D1 family recombinant expression”或“olfactory receptor OR8D4 functional study”扩展结果。
Recombinant human OR8D4 protein is a chemically synthesized or heterologously expressed form of the olfactory receptor OR8D4, a class A G protein-coupled receptor (GPCR) primarily found in olfactory sensory neurons. As part of the largest GPCR family, olfactory receptors like OR8D4 are responsible for detecting odorant molecules and initiating signal transduction cascades that translate chemical signals into neural responses. OR8D4 is localized on chromosome 11 and is notably responsive to specific odorants, though its exact endogenous ligands remain under investigation.
The recombinant version is typically produced using expression systems such as HEK293 cells or insect cell lines engineered to mimic native post-translational modifications, enabling functional studies. Production challenges include ensuring proper membrane localization and conformational stability due to GPCR complexity. Research on recombinant OR8D4 focuses on deciphering its ligand-binding specificity, activation mechanisms, and potential non-olfactory roles in tissues like the lungs or brain.
Its recombinant form has become critical for structural biology (e.g., cryo-EM or X-ray crystallography) and drug discovery platforms targeting olfactory-related disorders or GPCR signaling pathways. Recent studies also explore its utility in biosensor development for odorant detection. However, functional redundancy among olfactory receptors and incomplete signaling pathway reconstitution in vitro remain key limitations. Continued optimization of expression platforms and high-throughput screening methods aim to address these challenges, positioning recombinant OR8D4 as a valuable tool for both basic research and applied biotechnology.
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