| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PAF1 |
| Uniprot No | Q8N7H5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-531 aa |
| 活性数据 | MAPTIQTQAQREDGHRPNSHRTLPERSGVVCRVKYCNSLPDIPFDPKFITYPFDQNRFVQYKATSLEKQHKHDLLTEPDLGVTIDLINPDTYRIDPNVLLDPADEKLLEEEIQAPTSSKRSQQHAKVVPWMRKTEYISTEFNRYGISNEKPEVKIGVSVKQQFTEEEIYKDRDSQITAIEKTFEDAQKSISQHYSKPRVTPVEVMPVFPDFKMWINPCAQVIFDSDPAPKDTSGAAALEMMSQAMIRGMMDEEGNQFVAYFLPVEETLKKRKRDQEEEMDYAPDDVYDYKIAREYNWNVKNKASKGYEENYFFIFREGDGVYYNELETRVRLSKRRAKAGVQSGTNALLVVKHRDMNEKELEAQEARKAQLENHEPEEEEEEEMETEEKEAGGSDEEQEKGSSSEKEGSEDEHSGSESEREEGDRDEASDKSGSGEDESSEDEARAARDKEEIFGSDADSEDDADSDDEDRGQAQGGSDNDSDSGSNGGGQRSRSHSRSASPFPSGSEHSAQEDGSEAAASDSSEADSDSD |
| 分子量 | 86.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人PAF1蛋白的典型研究方向及摘要示例（文献信息为模拟数据）：
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1. **文献名称**: *Structural insights into human PAF1 complex assembly and chromatin interaction*
**作者**: Chen X, Li R et al. (2020)
**摘要**: 解析了重组人PAF1蛋白与其复合体成员（Cdc73、Leo1等）的三维结构，揭示了PAF1在组蛋白H2B泛素化中的调控机制，并通过体外实验证明其与RNA聚合酶II的互作对转录延伸至关重要。
2. **文献名称**: *PAF1 regulates stem cell pluripotency through histone modification crosstalk*
**作者**: Smith A, Kim H et al. (2018)
**摘要**: 利用重组人PAF1蛋白进行体外染色质结合实验，发现PAF1通过招募H3K4me3和H3K36me3修饰酶维持胚胎干细胞的自我更新能力，敲除PAF1导致多能性基因表达失调。
3. **文献名称**: *Development of a PAF1-based biosensor for epigenetic drug screening*
**作者**: Gonzalez L, Wei Y et al. (2022)
**摘要**: 基于重组人PAF1蛋白构建了一种荧光共振能量转移（FRET）探针，用于高通量筛选靶向PAF1复合体的小分子抑制剂，为癌症表观遗传治疗提供新工具。
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**注意**：以上文献为生成内容示例，实际文献需通过PubMed/Google Scholar等平台检索关键词"recombinant human PAF1"或"PAF1 complex"获取，推荐结合研究领域细化搜索（如转录调控、癌症机制等）。
The human Polymerase-Associated Factor 1 (PAF1) protein is a critical component of the PAF1 complex, a multifunctional transcriptional regulatory complex involved in diverse cellular processes, including transcription elongation, histone modification, mRNA surveillance, and chromatin remodeling. The PAF1 complex associates with RNA polymerase II (Pol II) and regulates gene expression by coordinating post-translational histone modifications such as H2B ubiquitination and H3K4/H3K36 methylation, which influence transcriptional activation and epigenetic memory. PAF1 itself plays a central role in stabilizing the complex and mediating interactions with other transcriptional cofactors.
Recombinant human PAF1 protein, produced via heterologous expression systems (e.g., *E. coli* or mammalian cells), retains its functional domains and structural integrity, enabling biochemical studies to dissect its molecular interactions and enzymatic roles. Its recombinant form is commonly used to investigate mechanisms of transcriptional regulation, DNA repair, stem cell maintenance, and carcinogenesis. Dysregulation of PAF1 has been linked to cancers and developmental disorders, highlighting its therapeutic potential.
Purified recombinant PAF1 facilitates *in vitro* assays, protein-protein interaction studies, and structural analyses (e.g., X-ray crystallography or Cryo-EM), offering insights into its role in disease pathways and its utility as a target for drug discovery.
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