| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PABPC3 |
| Uniprot No | Q9H361 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-631 aa |
| 活性数据 | MNPSTPSYPT ASLYVGDLHP DVTEAMLYEK FSPAGPILSI RICRDLITSG SSNYAYVNFQ HTKDAEHALD TMNFDVIKGK PVRIMWSQRD PSLRKSGVGN IFVKNLDKSI NNKALYDTVS AFGNILSCNV VCDENGSKGY GFVHFETHEA AERAIKKMNG MLLNGRKVFV GQFKSRKERE AELGARAKEF PNVYIKNFGE DMDDERLKDL FGKFGPALSV KVMTDESGKS KGFGFVSFER HEDAQKAVDE MNGKELNGKQ IYVGRAQKKV ERQTELKRTF EQMKQDRITR YQVVNLYVKN LDDGIDDERL RKAFSPFGTI TSAKVMMEGG RSKGFGFVCF SSPEEATKAV TEMNGRIVAT KPLYVALAQR KEERQAYLTN EYMQRMASVR AVPNQRAPPS GYFMTAVPQT QNHAAYYPPS QIARLRPSPR WTAQGARPHP FQNKPSAIRP GAPRVPFSTM RPASSQVPRV MSTQRVANTS TQTVGPRPAA AAAAAATPAV RTVPRYKYAA GVRNPQQHRN AQPQVTMQQL AVHVQGQETL TASRLASAPP QKQKQMLGER LFPLIQAMHP TLAGKITGML LEIDNSELLY MLESPESLRS KVDEAVAVLQ AHQAKEATQK AVNSATGVPT V |
| 分子量 | 70.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PABPC3蛋白的模拟参考文献示例（内容为模拟创作，非真实文献）：
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1. **文献名称**: *"Characterization of Recombinant Human PABPC3 and Its Role in mRNA Stability"*
**作者**: Smith A, et al.
**摘要**: 本研究利用大肠杆菌系统表达重组人PABPC3蛋白，纯化后通过体外RNA结合实验证实其与poly(A)尾的特异性结合。进一步研究发现，PABPC3可增强靶mRNA的稳定性，并通过与翻译起始因子相互作用调控蛋白合成。
2. **文献名称**: *"Tissue-Specific Expression and Functional Analysis of PABPC3 in Mammalian Germ Cells"*
**作者**: Tanaka K, et al.
**摘要**: 通过重组人PABPC3的真核表达系统（HEK293细胞），研究揭示了其在哺乳动物睾丸组织中的高表达特性。功能分析表明，PABPC3参与精子发生过程中的翻译调控，缺失会导致生殖细胞发育异常。
3. **文献名称**: *"Structural Insights into PABPC3’s RNA-Binding Domain by Recombinant Protein Crystallography"*
**作者**: Li X, et al.
**摘要**: 利用重组表达的PABPC3 RRM（RNA识别基序）结构域进行X射线晶体学研究，解析了其与poly(A) RNA复合物的三维结构，揭示了关键氨基酸残基在RNA结合中的作用机制。
4. **文献名称**: *"PABPC3 Dysregulation in Cancer: Implications for mRNA Translation Pathways"*
**作者**: Garcia R, et al.
**摘要**: 通过分析重组人PABPC3在多种癌细胞系中的过表达效应，发现其可异常激活致癌基因mRNA的翻译，提示其作为潜在肿瘤治疗靶点的可能性。
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**说明**：以上文献信息为模拟生成，实际引用时请核实真实文献并补充完整的期刊名称、卷号及页码。如需真实文献推荐，可通过PubMed或Google Scholar以“PABPC3”或“Polyadenylate-binding protein cytoplasmic 3”为关键词检索最新研究。
Poly(A)-binding protein cytoplasmic 3 (PABPC3) is a member of the poly(A)-binding protein (PABP) family, which plays critical roles in post-transcriptional regulation of gene expression. Unlike its ubiquitously expressed homolog PABPC1, PABPC3 exhibits tissue-specific expression, predominantly in the testis, suggesting a specialized function in spermatogenesis. It binds to the poly(A) tails of mRNAs, enhancing their stability and facilitating translation by interacting with eukaryotic initiation factors (eIFs) and the ribosome. Structurally, PABPC3 contains conserved RNA recognition motifs (RRMs) that mediate RNA binding and a proline-rich C-terminal domain involved in protein-protein interactions.
Recombinant human PABPC3 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Its recombinant form allows researchers to dissect molecular mechanisms underlying mRNA metabolism in germ cell development and infertility. Studies suggest PABPC3 may contribute to translational control during spermiogenesis, a phase marked by rapid protein synthesis in elongating spermatids. Dysregulation of PABPC3 has been implicated in male infertility, highlighting its clinical relevance. As a recombinant protein, it also serves as a tool for structural analyses, antibody production, and screening for small-molecule modulators of PABP activity. Further research on PABPC3 could uncover novel insights into reproductive biology and RNA regulation in specialized cellular contexts.
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