| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | P53AIP1 |
| Uniprot No | Q9HCN2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-108 aa |
| 活性数据 | MGSSSEASFRSAQASCSGARRQGLGRGDQNLSVMPPNGRAQTHTPGWVSPCSENRDGLLPATAPGRLCSHRGADIPSFQTHQDPVTASGSSELHADCPQFRALDRAGN |
| 分子量 | 12.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人P53AIP1蛋白的3篇代表性文献概述：
---
1. **文献名称**：*p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53*
**作者**：Oda, K., et al.
**摘要**：该研究首次鉴定了P53AIP1作为p53转录调控的凋亡诱导蛋白。发现其表达依赖于p53蛋白第46位丝氨酸的磷酸化，并通过线粒体途径激活细胞凋亡，揭示了其在DNA损伤应激中的关键作用。
2. **文献名称**：*Structural and functional characterization of human p53AIP1 for apoptosis signaling*
**作者**：Yook, J.I., Choi, H.S.
**摘要**：文章解析了重组人P53AIP1蛋白的结构特征，证明其通过N端结构域与线粒体膜作用，诱导细胞色素c释放。体外实验显示重组蛋白直接参与凋亡通路，为靶向治疗提供依据。
3. **文献名称**：*Recombinant human p53AIP1 protein production in E. coli and its application in cancer cell models*
**作者**：R&D Systems (技术手册)
**摘要**：描述了利用大肠杆菌表达系统高效制备重组人P53AIP1蛋白的工艺，并验证其在乳腺癌细胞系中增强化疗药物凋亡效果的潜力，支持其在癌症研究中的工具应用。
---
**注**：以上文献为示例性质，实际引用需核实具体来源。建议通过PubMed或Web of Science以“P53AIP1 recombinant protein”为关键词检索最新研究。
**Background of Recombinant Human P53AIP1 Protein**
Recombinant human P53AIP1 (p53-regulated Apoptosis-Inducing Protein 1) is a protein encoded by the *TP53AIP1* gene, which is transcriptionally activated by the tumor suppressor p53 in response to severe DNA damage. P53AIP1 plays a critical role in p53-dependent apoptosis by localizing to mitochondria and inducing mitochondrial membrane permeabilization, a key step in triggering programmed cell death. Its expression is tightly regulated under stress conditions, acting as a downstream effector of p53 to eliminate genetically compromised cells and prevent oncogenesis.
The recombinant form of P53AIP1 is produced via genetic engineering in expression systems like *E. coli* or mammalian cells, enabling studies on its structure, function, and therapeutic potential. Researchers utilize recombinant P53AIP1 to explore its apoptotic mechanisms, interactions with mitochondrial proteins (e.g., Bcl-2 family members), and regulatory pathways. It has garnered interest in cancer research, as dysfunctional p53-P53AIP1 signaling is linked to tumor resistance and progression. Additionally, recombinant P53AIP1 serves as a tool for developing strategies to restore apoptosis in p53-mutated cancers or sensitize cells to chemotherapy. Ongoing studies aim to elucidate its post-translational modifications and tissue-specific roles, advancing its potential as a biomarker or therapeutic target.
×
