| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARP6 |
| Uniprot No | Q2NL67 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-630 aa |
| 活性数据 | MDIKGQFWNDDDSEGDNESEEFLYGVQGSCAADLYRHPQLDADIEAVKEIYSENSVSIREYGTIDDVDIDLHINISFLDEEVSTAWKVLRTEPIVLRLRFSLSQYLDGPEPSIEVFQPSNKEGFGLGLQLKKILGMFTSQQWKHLSNDFLKTQQEKRHSWFKASGTIKKFRAGLSIFSPIPKSPSFPIIQDSMLKGKLGVPELRVGRLMNRSISCTMKNPKVEVFGYPPSPQAGLLCPQHVGLPPPARTSPLVSGHCKNIPTLEYGFLVQIMKYAEQRIPTLNEYCVVCDEQHVFQNGSMLKPAVCTRELCVFSFYTLGVMSGAAEEVATGAEVVDLLVAMCRAALESPRKSIIFEPYPSVVDPTDPKTLAFNPKKKNYERLQKALDSVMSIREMTQGSYLEIKKQMDKLDPLAHPLLQWIISSNRSHIVKLPLSRLKFMHTSHQFLLLSSPPAKEARFRTAKKLYGSTFAFHGSHIENWHSILRNGLVNASYTKLQLHGAAYGKGIYLSPISSISFGYSGMGKGQHRMPSKDELVQRYNRMNTIPQTRSIQSRFLQSRNLNCIALCEVITSKDLQKHGNIWVCPVSDHVCTRFFFVYEDGQVGDANINTQDPKIQKEIMRVIGTQVYTN |
| 分子量 | 78 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PARP6蛋白的参考文献示例(注：以下文献信息为示例性虚构，实际需通过学术数据库验证)：
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1. **文献名称**：**"PARP6 regulates cellular redox homeostasis and oxidative DNA damage repair"**
**作者**：Smith A et al.
**摘要**：本研究首次报道了重组人PARP6蛋白在氧化应激条件下的功能，发现其通过调节NAD+代谢和激活下游修复通路参与氧化性DNA损伤修复，并与PARP1功能存在协同作用。
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2. **文献名称**：**"Crystal structure of human PARP6 catalytic domain and insights into its substrate recognition"**
**作者**：Wang L et al.
**摘要**：通过X射线晶体学解析了重组人PARP6催化结构域的三维结构，揭示了其底物结合口袋的特征，并证明其偏向单链DNA结合的特性，为靶向抑制剂设计提供了结构基础。
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3. **文献名称**：**"PARP6 promotes cancer cell proliferation via non-canonical Wnt/β-catenin signaling"**
**作者**：Chen X et al.
**摘要**：研究利用重组PARP6蛋白及基因敲除模型，证明其通过调控非经典Wnt信号通路促进乳腺癌细胞增殖，并证明其酶活性依赖于单ADP核糖基转移功能。
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4. **文献名称**：**"Development of a high-throughput assay for screening PARP6 inhibitors using recombinant protein"**
**作者**：Zhang Y et al.
**摘要**：建立了基于重组人PARP6蛋白的高通量抑制剂筛选平台，筛选出小分子化合物库中具有潜在选择性的PARP6抑制剂，为靶向治疗神经退行性疾病提供新策略。
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**注意**：以上文献为简化示例，实际研究需通过PubMed(https://pubmed.ncbi.nlm.nih.gov)或Web of Science等平台检索真实文献。推荐关键词组合：**"PARP6 recombinant protein" + "structure/function/inhibitor"**。
Recombinant human PARP6 (poly(ADP-ribose) polymerase 6) is a member of the PARP family, which plays diverse roles in cellular processes such as DNA repair, genomic stability, and signal transduction. PARP6. also known as ARTD17. belongs to the "mono-ADP-ribosyltransferase" subfamily, distinct from classical PARPs involved in poly-ADP-ribosylation. It catalyzes the transfer of a single ADP-ribose unit to target proteins, modulating their activity or interactions. Structurally, PARP6 contains a conserved catalytic PARP domain and a unique N-terminal domain of unknown function.
Research suggests PARP6 regulates microtubule dynamics, cell cycle progression, and cellular stress responses. It is implicated in cancer, where aberrant expression correlates with tumor progression, metastasis, and drug resistance. For instance, PARP6 overexpression has been observed in breast and ovarian cancers, potentially influencing oncogenic signaling pathways.
Recombinant PARP6 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies and drug discovery. Its enzymatic activity is often assessed in vitro using NAD⁺-dependent ADP-ribosylation assays. Inhibitors targeting PARP6 are under exploration to develop cancer therapies with fewer side effects than pan-PARP inhibitors. Despite progress, the full scope of PARP6's biological roles and substrate specificity remains under investigation, highlighting its emerging significance in molecular oncology and cell biology.
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