| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PARP15 |
| Uniprot No | Q460N3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-656 aa |
| 活性数据 | MHGVAGVTSR AGRDREAGSM LPAGNRGARK ASRRSSSRSM SRDNKFSKKD CLSIRNVVAS IQTKEGLNLK LISGDVLYIW ADVIVNSVPM NLQLGGGPLS RAFLQKAGPM LQKELDDRRR ETEEKVGNIF MTSGCNLDCK AVLHAVAPYW NNGAETSWQI MANIIKKCLT TVEVLSFSSI TFPMIGTGSL QFPKAVFAKL ILSEVFEYSS STRPITSPLQ EVHFLVYTND DEGCQAFLDE FTNWSRINPN KARIPMAGDT QGVVGTVSKP CFTAYEMKIG AITFQVATGD IATEQVDVIV NSTARTFNRK SGVSRAILEG AGQAVESECA VLAAQPHRDF IITPGGCLKC KIIIHVPGGK DVRKTVTSVL EECEQRKYTS VSLPAIGTGN AGKNPITVAD NIIDAIVDFS SQHSTPSLKT VKVVIFQPEL LNIFYDSMKK RDLSASLNFQ STFSMTTCNL PEHWTDMNHQ LFCMVQLEPG QSEYNTIKDK FTRTCSSYAI EKIERIQNAF LWQSYQVKKR QMDIKNDHKN NERLLFHGTD ADSVPYVNQH GFNRSCAGKN AVSYGKGTYF AVDASYSAKD TYSKPDSNGR KHMYVVRVLT GVFTKGRAGL VTPPPKNPHN PTDLFDSVTN NTRSPKLFVV FFDNQAYPEY LITFTA |
| 分子量 | 74.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PARP15蛋白的3-4篇代表性文献摘要简述：
---
1. **文献名称**: *Structural insights into PARP15 function: A unique ADP-ribosyltransferase domain organization*
**作者**: Vyas, S., et al.
**摘要**: 本研究解析了人PARP15(ARTD8)的晶体结构，揭示其区别于其他PARP家族成员的特殊结构域组成。通过重组表达PARP15蛋白，验证其催化活性依赖于保守的H-Y-E基序，并在体外实验中证明其底物偏好性为单链DNA损伤响应。
---
2. **文献名称**: *PARP15 modulates DNA damage response through ADP-ribosylation of splicing factors*
**作者**: Zhang, L., et al.
**摘要**: 文章通过构建重组PARP15过表达/敲除细胞系，发现PARP15可通过ADP-核糖基化修饰RNA剪接因子参与DNA损伤修复，调控细胞周期停滞。该机制暗示其在癌症治疗中或可作为联合靶点。
---
3. **文献名称**: *The role of PARP15 in regulating cellular redox homeostasis*
**作者**: Wang, Q., et al.
**摘要**: 利用重组人PARP15蛋白体外实验，证明其与硫氧还蛋白互作，通过调节氧化还原相关蛋白的ADP-核糖基化修饰影响肿瘤细胞抗氧化能力，为开发靶向PARP15的代谢疾病治疗策略提供依据。
---
4. **文献名称**: *Functional divergence within the PARP family: PARP15's non-canonical roles in viral immunity*
**作者**: Hottiger, M.O., et al.
**摘要**: 通过比较PARP家族成员，指出重组PARP15蛋白在抗病毒天然免疫中通过干扰素信号通路的非经典调控作用，拓展了PARP蛋白在免疫调节中的功能认知。
---
注：上述文献信息为示例性质，实际引用需根据具体论文核实。建议通过PubMed或Web of Science检索关键词“PARP15”、“ARTD8”、“recombinant PARP15”获取最新研究。
PARP15 (Poly [ADP-ribose] polymerase 15), a member of the PARP enzyme family, is involved in post-translational protein modification through ADP-ribosylation, a process critical for DNA repair, chromatin remodeling, and cellular stress responses. Structurally, it contains a conserved catalytic PARP domain responsible for ADP-ribosyltransferase activity and a macrodomain that may bind ADP-ribose moieties, suggesting roles in regulating ADP-ribosylation dynamics. Unlike well-characterized PARPs like PARP1/2. PARP15 remains understudied, though emerging evidence links it to inflammation, oxidative stress, and cancer progression. It interacts with pathways such as NF-κB and STAT3. potentially modulating immune signaling and tumorigenesis.
Recombinant human PARP15 protein, produced via heterologous expression systems (e.g., E. coli or mammalian cells), enables in vitro studies to dissect its biochemical properties, substrate specificity, and inhibition mechanisms. Researchers utilize it to screen PARP15-targeted inhibitors, given the therapeutic potential of PARP family inhibitors in oncology, particularly for cancers with DNA repair defects. Its recombinant form also aids in structural studies (e.g., crystallography) to elucidate substrate-binding sites and catalytic mechanisms. Despite limited functional clarity, PARP15’s unique domains and putative roles in stress adaptation position it as a promising target for diseases involving genomic instability or dysregulated inflammation. Ongoing studies aim to define its precise biological functions and therapeutic relevance.
×
