| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHGC3 |
| Uniprot No | Q9UN70 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-934 aa |
| 活性数据 | MVPEAWRSGLVSTGRVVGVLLLLGALNKASTVIHYEIPEEREKGFAVGNVVANLGLDLGSLSARRFRVVSGASRRFFEVNRETGEMFVNDRLDREELCGTLPSCTVTLELVVENPLELFSVEVVIQDINDNNPAFPTQEMKLEISEAVAPGTRFPLESAHDPDVGSNSLQTYELSRNEYFALRVQTREDSTKYAELVLERALDREREPSLQLVLTALDGGTPALSASLPIHIKVLDANDNAPVFNQSLYRARVLEDAPSGTRVVQVLATDLDEGPNGEIIYSFGSHNRAGVRQLFALDLVTGMLTIKGRLDFEDTKLHEIYIQAKDKGANPEGAHCKVLVEVVDVNDNAPEITVTSVYSPVPEDAPLGTVIALLSVTDLDAGENGLVTCEVPPGLPFSLTSSLKNYFTLKTSADLDRETVPEYNLSITARDAGTPSLSALTIVRVQVSDINDNPPQSSQSSYDVYIEENNLPGAPILNLSVWDPDAPQNARLSFFLLEQGAETGLVGRYFTINRDNGIVSSLVPLDYEDRREFELTAHISDGGTPVLATNISVNIFVTDRNDNAPQVLYPRPGGSSVEMLPRGTSAGHLVSRVVGWDADAGHNAWLSYSLLGSPNQSLFAIGLHTGQISTARPVQDTDSPRQTLTVLIKDNGEPSLSTTATLTVSVTEDSPEARAEFPSGSAPREQKKNLTFYLLLSLILVSVGFVVTVFGVIIFKVYKWKQSRDLYRAPVSSLYRTPGPSLHADAVRGGLMSPHLYHQVYLTTDSRRSDPLLKKPGAASPLASRQNTLRSCDPVFYRQVLGAESAPPGQQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 分子量 | 128.48 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于原钙粘蛋白家族研究的假设性参考文献(注：PCDHGC3相关研究较少，建议通过PubMed或Google Scholar核实具体文献)：
1. **文献名称**：Structural characterization of recombinant human Protocadherin-Gamma-C3 (PCDHGC3) extracellular domain
**作者**：Li, X. et al.
**摘要**：本研究利用哺乳动物表达系统重组表达了人PCDHGC3蛋白的胞外结构域，并通过X射线晶体学解析其三维结构，揭示了其独特的钙离子依赖型同源二聚化机制，为原钙粘蛋白介导的细胞间相互作用提供了结构基础。
2. **文献名称**：PCDHGC3 regulates neuronal differentiation via Wnt/β-catenin signaling
**作者**：Wang, Y. et al.
**摘要**：通过重组PCDHGC3蛋白的功能实验，发现其在神经干细胞分化过程中通过调控β-catenin的核转位激活Wnt信号通路，表明其在神经发育中的潜在作用。
3. **文献名称**：Development of a high-yield prokaryotic expression system for human PCDHGC3
**作者**：Chen, J. et al.
**摘要**：构建了基于大肠杆菌的重组PCDHGC3蛋白表达体系，优化诱导条件后获得毫克级可溶性蛋白，经纯化验证可用于后续抗体制备及功能分析。
**建议**：由于PCDHGC3属非经典原钙粘蛋白亚家族，实际研究可能较少。推荐使用关键词"PCDHGC3"或"protocadherin gamma subfamily C3"在**Web of Science**或**NCBI PubMed**进行文献精确定位，并关注近年神经发育或癌症相关研究。
**Background of Recombinant Human PCDHGC3 Protein**
Protocadherin gamma subfamily C3 (PCDHGC3) belongs to the non-clustered protocadherin γ family, a subgroup of the cadherin superfamily. These transmembrane proteins are characterized by extracellular cadherin-like repeats that mediate calcium-dependent cell-cell adhesion. The human PCDHGC3 gene, located on chromosome 5q31. encodes a single-pass transmembrane protein with seven extracellular cadherin domains and a conserved cytoplasmic domain. Unlike classical cadherins, protocadherins like PCDHGC3 are highly expressed in the nervous system and play critical roles in neuronal circuit formation, synaptic plasticity, and axonal guidance.
Recombinant PCDHGC3 is artificially produced using molecular cloning techniques, often in mammalian or insect cell systems, to ensure proper post-translational modifications. Its study aids in elucidating mechanisms underlying neural development and disorders such as autism, schizophrenia, and epilepsy, where protocadherin dysfunction has been implicated. Additionally, PCDHGC3 may interact with intracellular signaling molecules to regulate cytoskeletal dynamics or synaptic connectivity. Recent research also explores its potential role in cancer, as abnormal protocadherin expression correlates with tumor progression and metastasis.
The availability of recombinant PCDHGC3 facilitates structural studies, antibody development, and high-throughput screening for therapeutic agents targeting protocadherin-related pathologies.
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