| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHGA12 |
| Uniprot No | O60330 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-932 aa |
| 活性数据 | MIPARLHRDYKGLVLLGILLGTLWETGCTQIRYSVPEELEKGSRVGDISRDLGLEPRELAERGVRIIPRGRTQLFALNPRSGSLVTAGRIDREELCMGAIKCQLNLDILMEDKVKIYGVEVEVRDINDNAPYFRESELEIKISENAATEMRFPLPHAWDPDIGKNSLQSYELSPNTHFSLIVQNGADGSKYPELVLKRALDREEKAAHHLVLTASDGGDPVRTGTARIRVMVLDANDNAPAFAQPEYRASVPENLALGTQLLVVNATDPDEGVNAEVRYSFRYVDDKAAQVFKLDCNSGTISTIGELDHEESGFYQMEVQAMDNAGYSARAKVLITVLDVNDNAPEVVLTSLASSVPENSPRGTLIALLNVNDQDSEENGQVICFIQGNLPFKLEKSYGNYYSLVTDIVLDREQVPSYNITVTATDRGTPPLSTETHISLNVADTNDNPPVFPQASYSAYIPENNPRGVSLVSVTAHDPDCEENAQITYSLAENTIQGASLSSYVSINSDTGVLYALSSFDYEQFRDLQVKVMARDNGHPPLSSNVSLSLFVLDQNDNAPEILYPALPTDGSTGVELAPRSAEPGYLVTKVVAVDRDSGQNAWLSYRLLKASEPGLFSVGLHTGEVRTARALLDRDALKQSLVVAVQDHGQPPLSATVTLTVAVADSIPQVLADLGSLESPANSETSDLTLYLVVAVAAVSCVFLAFVILLLALRLRRWHKSRLLQASGGGLTGAPASHFVGVDGVQAFLQTYSHEVSLTTDSRKSHLIFPQPNYADMLVSQESFEKSEPLLLSGDSVFSKDSHGLIEQAPPNTDWRFSQAQRPGTSGSQNGDDTGTWPNNQFDTEMLQAMILASASEAADGSSTLGGGAGTMGLSARYGPQFTLQHVPDYRQNVYIPGSNATLTNAAGKRDGKAPAGGNGNKKKSGKKEKK |
| 分子量 | 127.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCDHGA12蛋白的虚构参考文献(仅为示例，非真实文献)：
1. **文献名称**：Expression and Functional Characterization of Recombinant Human PCDHGA12 in Mammalian Cells
**作者**：Zhang L., et al.
**摘要**：本研究成功在HEK293细胞中表达了重组人PCDHGA12蛋白，通过亲和层析纯化获得高纯度蛋白。功能实验表明，PCDHGA12通过钙离子依赖性细胞黏附途径参与神经元突触的形成，提示其在神经系统发育中的作用。
2. **文献名称**：Structural Insights into the Extracellular Domain of PCDHGA12 by Cryo-EM
**作者**：Smith J.R., et al.
**摘要**：利用冷冻电镜解析了重组PCDHGA12蛋白胞外区的三维结构，揭示了其独特的钙结合结构域构象，并探讨了该结构如何影响同源/异源原钙粘蛋白间的相互作用及信号转导。
3. **文献名称**：Recombinant PCDHGA12 Suppresses Tumor Metastasis via Regulating Wnt/β-catenin Pathway
**作者**：Wang Y., et al.
**摘要**：体外实验显示，外源性添加重组PCDHGA12蛋白可抑制乳腺癌细胞迁移，机制与下调Wnt/β-catenin通路及减少上皮间质转化(EMT)标志物表达相关，提示其潜在抗癌应用价值。
4. **文献名称**：High-throughput Screening of PCDHGA12-binding Peptides for Neural Regeneration
**作者**：Lee S., et al.
**摘要**：通过噬菌体展示技术筛选出与重组PCDHGA12特异性结合的多肽，证实其可促进体外神经元轴突生长，为神经损伤修复提供了新的分子工具。
注：以上内容为模拟生成，实际文献需通过学术数据库检索确认。
Protocadherin gamma-A12 (PCDHGA12) is a member of the protocadherin γ gene cluster, a subgroup of the cadherin superfamily involved in cell-cell adhesion and neural circuit assembly. Located on human chromosome 5q31. this transmembrane protein contains seven extracellular cadherin repeats, a transmembrane domain, and a cytoplasmic tail with conserved motifs for intracellular signaling. PCDHGA12 exhibits calcium-dependent homophilic binding properties, contributing to neuronal diversity, synaptic specificity, and brain development through selective cell recognition mechanisms.
As a clustered protocadherin, it plays roles in axon guidance, dendritic arborization, and neuronal self-avoidance. Dysregulation has been tentatively linked to neurodevelopmental disorders and cancer progression, though mechanistic details remain understudied. Recombinant human PCDHGA12 protein, typically produced via mammalian or insect cell systems, retains functional domains for structural studies or interaction assays. Its modular design allows exploration of adhesion kinetics, synapse formation models, and potential therapeutic targeting. Current research focuses on deciphering its isoform-specific roles in neural plasticity and tumor suppression, leveraging recombinant forms to map binding partners and signaling cascades.
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