| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHB11 |
| Uniprot No | Q9Y5F2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MENQGTRTQQIRQVLLLFVLLGMSQAGSETWSFSVAEEMQSGSFVGNLAKDLGLKVRELSSRGARVVSNDKKQRLQLDINTGDLLLSETLDREELCGSIEPCVLHLQVLMQNPTQFLQIELQVRDINDHSPIFSEKQMLLEIPENSPVGAVFLLESAKDLDVGINAVKSYTISPNSHFHIKMRVIPDNRKYPELVLDKALDYEELPELSFILSALDGGSPPRSGTALVRVVVVDINDNSPEFEQAFYEVKIRENSILGSLILIVSAWDLDSGTNGEICYTFSHASEDIRKTFEINQKSGEITLRAPLDFETIESYSIIIQATDGGGLFGKSTVIIHVIDVNDNAPEITVSSITSPIPENTPETVVMVFSIQDIDSGDNGRIVCSIPEDLPFVLKSSVENYYTLETERPLDRESTAEYNITITVTDLGIPRLKTEHNTTVLVSDVNDNAPTFTQTSYTLFVRENNSPALHIGSVSATDRDSGTNAQVNYSLLPPQDLHLPLASLVSINTDNGHLFALRSLDYEALQAFDFRVGATDRGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPRAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLQVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAASVGSCSVPKGPFPGHLVDVSGTGTLSQSYQYEVCLTGGSETNEFKFLKPVIPNIQAKGLGKNSEENSTFRNSFGFNF |
| 分子量 | 87.7 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCDHB11蛋白的3篇示例文献(注：部分文献为示例性概括，具体研究需查询真实数据库)：
1. **文献名称**：*"Recombinant expression and functional characterization of human PCDHB11 in neural development"*
**作者**：Smith J, et al.
**摘要**：研究团队通过大肠杆菌系统成功表达并纯化重组人PCDHB11蛋白，发现其在体外神经元培养中促进轴突生长和突触形成，提示其在神经发育中的潜在作用。
2. **文献名称**：*"Structural insights into PCDHB11 interactions with extracellular matrix proteins"*
**作者**：Chen L, et al.
**摘要**：通过X射线晶体学解析重组PCDHB11蛋白结构，揭示其胞外域与层粘连蛋白的结合特性，为原钙粘蛋白家族参与细胞黏附的分子机制提供证据。
3. **文献名称**：*"PCDHB11 gene variants and altered protein expression in autism spectrum disorders"*
**作者**：Kim S, et al.
**摘要**：分析自闭症患者脑组织样本，发现PCDHB11表达水平显著下调。体外实验表明，重组PCDHB11蛋白缺失导致突触功能异常，提示其与神经发育疾病的相关性。
**注意**：以上文献为基于领域研究的示例，实际文献需通过PubMed、Google Scholar等数据库检索关键词“recombinant PCDHB11”或“PCDHB11 function”获取。部分研究可能需结合原钙粘蛋白家族(protocadherin)的广义文献进行推导。
**Background of Recombinant Human PCDHB11 Protein**
Protocadherin beta-11 (PCDHB11) is a member of the protocadherin β cluster within the cadherin superfamily, which plays critical roles in cell-cell adhesion and synaptic specificity in the nervous system. Encoded by the *PCDHB11* gene on chromosome 5q31. this transmembrane protein features six extracellular cadherin repeats, a single-pass transmembrane domain, and a short cytoplasmic tail with conserved motifs for intracellular signaling. Unlike classical cadherins, clustered protocadherins like PCDHB11 exhibit combinatorial diversity through stochastic promoter selection, enabling precise neuronal identity and connectivity patterns during brain development.
PCDHB11 is predominantly expressed in the central nervous system, where it contributes to axon guidance, dendritic arborization, and synapse formation. Its homophilic or heterophilic binding properties mediate selective neuronal recognition, facilitating self-avoidance and neural circuit assembly. Dysregulation of protocadherins has been linked to neurodevelopmental disorders, including autism spectrum disorders and schizophrenia, though the specific role of PCDHB11 in these conditions remains under investigation.
Recombinant human PCDHB11 protein, produced via mammalian or bacterial expression systems, is utilized for structural studies, ligand-receptor interaction assays, and functional analyses in neuronal models. It serves as a tool to explore molecular mechanisms underlying neural development and synaptic plasticity, as well as potential therapeutic targets for neurological diseases.
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