| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHAC2 |
| Uniprot No | Q9Y5I4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-884 aa |
| 活性数据 | MEQAGTRPAATEHPRLRRPMPWLLLLPLLLLLLLLLPGPAASQLRYSVPEEQAPGALVGNVARALGLELRRLGPGCLRINHLGAPSPRYLELDLTSGALFVNERIDREALCEQRPRCLLSLEVLAHNPVAVSAVEVEILDINDNSPRFPRPNYQLQVSESVAPGARFHIESAQDPDVGANSVQTYELSPSEHFELDLKPLQENSKVLELVLRKGLDREQAALHHLVLTAVDGGIPARSGTAQISVRVLDTNDNSPAFDQSTYRVQLREDSPPGTLVVKLNASDPDEGSNGELRYSLSSYTSDRERQLFSIDASTGEVRVIGGLDYEEASSYQIYVQATDRGPVPMAGHCKVLVDIVDVNDNAPEVVLTDLYSPVPENATPNTIVAVLSVNDQDSGPNRKVSLGLEATLPFRLNGFGNSYTLVVSGPLDRERVAVYNITVTATDGGIPQLTSLRTLKVEISDINDNPPSFLEDSYSIYIQENNLPGVLLCTVQATDPDEKENAEVTYSLLEREIQGLPVTSYVSINSASGSLYAVNSFDYEKFREFFVTVEAQDKGSPPLSSTVTANVYVVDMNDHAPHILYPTSTNSSAAFEMVPRTAPAGYLVTKVIAMDSDSGQNAWLFYHLAQTSDLDLFKVELHTGEIRTTRKMGDESGSTFNLTVVVRDNGEPSLSASVAITVAVVDRVSKILPDTQRHVKSPRTYSEITLYLIIALSTVSFIFLLTIIILSIIKCYRYTAYGTACCGGFCGVRERSPAELYKQANNNIDARIPHGLKVQPHFIEVRGNGSLTKTYCYKACLTAGSGSDTFMFYNTGAQTGPGPSGAQAAVTDSRNLTGQSGQNAGNLIILKNEAVSQNEVRQWSGGLLQTHAFVTHPPISCDLALLSH |
| 分子量 | 122.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCDHAC2蛋白的3篇代表性文献的简要信息(注：因文献数据库访问限制，以下内容综合了类似研究的典型方向，具体文献可能需通过学术平台检索验证)：
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1. **文献名称**：*"Cloning and expression of human PCDHAC2 in HEK293 cells: implications for neural cell adhesion"*
**作者**：Li, X., Wang, Y., & Zhang, H.
**摘要**：该研究成功构建了人源PCDHAC2基因的重组表达载体，并在HEK293细胞中实现高产量表达。通过Western blot和免疫荧光验证了重组蛋白的细胞膜定位，初步功能实验表明其在神经细胞黏附中可能起调控作用。
2. **文献名称**：*"Structural characterization of recombinant PCDHAC2 extracellular domain and its role in cancer cell migration"*
**作者**：Chen, J., Liu, R., & Tanaka, K.
**摘要**：报道了重组表达并纯化PCDHAC2的胞外结构域(ECD)，通过晶体学分析其三维结构。功能研究表明，该蛋白通过调控MAPK信号通路抑制肺癌细胞的迁移能力，提示其潜在的肿瘤抑制功能。
3. **文献名称**：*"Proteolytic processing of recombinant PCDHAC2 in neuronal development"*
**作者**：Kim, S., Park, J., & Lee, M.
**摘要**：探讨重组PCDHAC2蛋白在小鼠神经元分化中的加工过程，发现其被caspase家族蛋白酶切割后释放的胞内片段可促进突触形成，强调其在神经发育中的动态调控机制。
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**注意**：以上文献标题及内容为模拟示例，具体研究请通过PubMed/Google Scholar以实际检索关键词(如“PCDHAC2 recombinant”)获取。原钙黏蛋白(protocadherin)家族的研究多集中于神经发育和疾病机制，重组表达技术常采用哺乳动物细胞系统或昆虫细胞-杆状病毒系统。
Protocadherin AC2 (PCDHAC2), a member of the non-clustered protocadherin family, is a calcium-dependent cell adhesion protein primarily expressed in the central nervous system. It plays critical roles in neuronal development, synaptic specificity, and neural circuit formation by mediating homophilic cell-cell interactions. Structurally, it contains seven extracellular cadherin repeats, a transmembrane domain, and a conserved cytoplasmic domain involved in intracellular signaling. Unlike classical cadherins, protocadherins like PCDHAC2 exhibit combinatorial diversity, contributing to neural complexity and individual cellular identity.
Recombinant human PCDHAC2 is typically produced using mammalian expression systems (e.g., HEK293 cells) to ensure proper post-translational modifications and functional integrity. Its purified form enables studies on molecular mechanisms underlying neurodevelopment, axon guidance, and synaptic plasticity. Emerging research links PCDHAC2 dysregulation to neuropsychiatric disorders, including autism spectrum disorders and schizophrenia, making it a potential biomarker or therapeutic target. Recent structural studies of recombinant PCDHAC2 have provided insights into its homodimerization interface and calcium-binding motifs.
Current applications span basic neuroscience research, drug screening platforms, and investigations into neural regeneration strategies. Challenges remain in fully characterizing its isoform-specific functions and interaction networks. As a recombinant tool, it significantly advances understanding of neural wiring specificity and developmental neuropathologies.
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