| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHB12 |
| Uniprot No | Q9Y5F1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-795 aa |
| 活性数据 | MENGGAGTLQIRQVLLFFVLLGMSQAGSETGNFLVMEELQSGSFVGNLAKTLGLEVSELSSRGARVVSNDNKECLQLDTNTGDLLLREMLDREELCGSNEPCVLYFQVLMKNPTQFLQIELQVRDINDHSPVFLEKEMLLEIPENSPVGAVFLLESAKDLDVGINAVKSYTINPNSHFHVKIRVNPDNRKYPELVLDKALDYEERPELSFILTALDGGSPPRSGTALVRVVVVDINDNSPEFEQAFYEVKILENSILGSLVVTVSAWDLDSGTNSELSYTFSHASEDIRKTFEINQKSGDITLTAPLDFEAIESYSIIIQATDGGGLFGKSTVRIQVMDVNDNAPEITVSSITSPIPENTPETVVMVFRIRDRDSGDNGKMVCSIPEDIPFVLKSSVNNYYTLETERPLDRESRAEYNITITVTDLGTPRLKTEHNITVLVSDVNDNAPAFTQTSYALFVRENNSPALHIGSISATDRDSGTNAQVNYSLLPSQDPHLPLASLVSINADNGHLFALRSLDYEALQGFQFRVGATDHGSPALSSEALVRVLVLDANDNSPFVLYPLQNGSAPCTELVPWAAEPGYLVTKVVAVDGDSGQNAWLSYQLLKATEPGLFGVWAHNGEVRTARLLSERDAAKHRLVVLVKDNGEPPRSATATLHVLLVDGFSQPYLPLPEAAPAQAQADSLTVYLVVALASVSSLFLFSVLLFVAVRLCRRSRAAPVGRCSVPEGPFPGHLVDVSGTGTLSQSYHYEVCVTGGSRSNKFKFLKPIIPNFLPQSTGSEVEENPPFQNNLGF |
| 分子量 | 113.2 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于公开信息模拟的关于重组人PCDHB12蛋白的参考文献示例(注：部分内容可能为虚构，实际文献需通过学术数据库验证):
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1. **标题**: "Expression and functional analysis of recombinant human PCDHB12 in neural adhesion"
**作者**: Zhang Y, et al. (2020)
**摘要**: 本研究利用大肠杆菌系统成功表达并纯化了重组人PCDHB12蛋白，证明其通过钙离子依赖的机制促进神经细胞黏附，为研究原钙黏蛋白在神经系统中的作用提供了工具。
2. **标题**: "Structural insights into PCDHB12 binding specificity by crystallography"
**作者**: Wang X, et al. (2021)
**摘要**: 通过X射线晶体学解析了PCDHB12胞外域的3D结构，揭示了其特异性结合细胞表面糖链的分子机制，为设计靶向神经疾病的药物奠定结构基础。
3. **标题**: "PCDHB12 modulates Wnt signaling pathway in glioblastoma cells"
**作者**: Li H, et al. (2018)
**摘要**: 发现重组PCDHB12通过激活Wnt/β-catenin通路增强胶质母细胞瘤细胞的迁移能力，提示其可能作为癌症治疗的新靶点。
4. **标题**: "Recombinant PCDHB12 inhibits tumor progression in vivo"
**作者**: Chen R, et al. (2019)
**摘要**: 在小鼠模型中，外源性重组PCDHB12显著抑制乳腺癌转移，可能通过竞争性阻断原钙黏蛋白介导的细胞间相互作用实现。
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**注意**: PCDHB12因研究较少，实际文献可能有限。建议通过PubMed或Google Scholar以“PCDHB12 recombinant”或“PCDHB12 protein function”为关键词检索最新研究，或扩展检索原钙黏蛋白家族(protocadherin)相关文献。
Recombinant human PCDHB12 protein belongs to the protocadherin-beta (Pcdhβ) subfamily within the cadherin superfamily, which plays critical roles in cell-cell adhesion and signaling. Protocadherins are transmembrane proteins predominantly expressed in the central nervous system, where they contribute to neuronal diversity, synaptic specificity, and neural circuit assembly. PCDHB12. encoded by the PCDHB12 gene located on chromosome 5q31. is one of 15 clustered β-protocadherins characterized by variable extracellular cadherin repeats and conserved cytoplasmic domains. These proteins mediate homophilic interactions, enabling precise neuronal connectivity during development.
The recombinant form of PCDHB12 is typically produced in vitro using expression systems (e.g., mammalian or bacterial cells) for functional studies. Research highlights its involvement in axon guidance, dendritic arborization, and synapse formation, potentially through interactions with intracellular signaling molecules like WAVE complex components. Dysregulation of PCDHB12 has been implicated in neurodevelopmental disorders and cancer progression, though its exact mechanistic pathways remain under investigation. Recombinant PCDHB12 serves as a valuable tool for structural analysis, ligand-receptor interaction assays, and exploring its role in neural network establishment or disease pathology, offering insights for therapeutic targeting.
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