| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCDHGB4 |
| Uniprot No | Q9UN71 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 0 |
| 活性数据 | MGSGAGELGRAERLPVLFLFLLSLFCPALCEQIRYRIPEEMPKGSVVGNLATDLGFSVQELPTRKLRVSSEKPYFTVSAESGELLVSSRLDREEICGKKPACALEFEAVAENPLNFYHVNVEIEDINDHTPKFTQNSFELQISESAQPGTRFILGSAHDADIGSNTLQNYQLSPSDHFSLINKEKSDGSKYPEMVLKTPLDREKQKSYHLTLTALDFGAPPLSSTAQIHVLVTDANDNAPVFSQDVYRVSLSENVYPGTTVLQVTATDQDEGVNAEITFSFSEASQITQFDLNSNTGEITVLNTLDFEEVKEYSIVLEARDGGGMIAQCTVEVEVIDENDNAPEVIFQSLPNLIMEDAELGTHIALLKVRDKDSRHNGEVTCKLEGDVPFKILTSSRNTYKLVTDAVLDREQNPEYNITVTATDRGKPPLSSSSSITLHIGDVNDNAPVFSQSSYIVHVAENNPPGASISQVRASDPDLGPNGQVSYCIMASDLEQRELSSYVSISAESGVVFAQRAFDHEQLRAFELTLQARDQGSPALSANVSLRVLVDDRNDNAPRVLYPALGPDGSALFDMVPHAAEPGYLVTKVVAVDADSGHNAWLSYHVLQASEPGLFSLGLRTGEVRTARALGDRDAVRQRLLVAVRDGGQPPLSATATLHLVFADSLQEVLPDITDRPDPSDLQAELQFYLVVALALISVLFLVAMILAIALRLRRSSSPASWSCFQPGLCVKSESVVPPNYSEGTLPYSYNLCVAHTGKTEFNFLKCSEQLSSGQDILCGDSSGALFPLCNSSELTSHQVSFL |
| 分子量 | 88.4 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCDHGB4蛋白的模拟参考文献示例(注：实际文献可能需要根据具体研究补充，以下内容为假设性概括)：
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1. **文献名称**: *"Cloning and Expression of Recombinant Human PCDHGB4 in Mammalian Cells"*
**作者**: Zhang L, et al.
**摘要**: 本研究成功构建了人PCDHGB4基因的真核表达载体，并在HEK293细胞中实现重组蛋白的高效表达。通过亲和层析纯化获得高纯度蛋白，Western blot验证其特异性。研究为后续功能研究提供了工具。
2. **文献名称**: *"Structural Insights into PCDHGB4: Role in Neural Cell Adhesion"*
**作者**: Smith J, et al.
**摘要**: 通过X射线晶体学解析了重组PCDHGB4蛋白的胞外结构域，揭示其钙依赖性同源二聚化机制。功能实验表明，PCDHGB4可能通过调节神经元突触粘附影响脑发育。
3. **文献名称**: *"PCDHGB4 Dysregulation in Cancer: Recombinant Protein-based Functional Analysis"*
**作者**: Tanaka K, et al.
**摘要**: 利用重组PCDHGB4蛋白进行体外实验，发现其过表达可抑制肿瘤细胞迁移。研究表明，PCDHGB4可能通过Wnt/β-catenin通路发挥抑癌作用，为癌症治疗提供新靶点。
4. **文献名称**: *"Proteomic Interaction Mapping of PCDHGB4 in the Human Brain"*
**作者**: Müller R, et al.
**摘要**: 采用重组PCDHGB4蛋白进行免疫共沉淀-质谱分析，鉴定出与其相互作用的突触后膜蛋白(如NLGN1)。结果提示PCDHGB4可能在神经回路形成中起关键作用。
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**提示**：以上为模拟摘要，实际文献需通过PubMed或Google Scholar检索关键词“PCDHGB4 recombinant”“protocadherin gamma B4”获取。原钙黏蛋白家族的功能研究较多，但针对特定亚型(如PCDHGB4)的研究可能有限，可扩大检索至相关家族或疾病模型。
**Background of Recombinant Human PCDHGB4 Protein**
Recombinant human PCDHGB4 (protocadherin gamma subfamily B4) is a member of the protocadherin family, a subset of the cadherin superfamily. Protocadherins are transmembrane proteins involved in cell-cell adhesion, neural circuit assembly, and synaptic specificity, playing critical roles in nervous system development and function. The γ-protocadherins, encoded by the *PCDHG* gene cluster, are particularly important for neuronal survival, axonal guidance, and synaptic diversity.
PCDHGB4 contains multiple extracellular cadherin domains, a transmembrane region, and a cytoplasmic tail. Its extracellular domains facilitate homophilic interactions, enabling selective recognition and adhesion between neurons. The cytoplasmic domain interacts with intracellular signaling proteins, modulating downstream pathways.
Recombinant PCDHGB4 is engineered using expression systems (e.g., mammalian or insect cells) to produce a purified, functional protein. It serves as a vital tool for studying mechanisms underlying neural development, including axon pathfinding, synaptic connectivity, and neuronal self-avoidance. Dysregulation of γ-protocadherins is linked to neurological disorders, such as epilepsy and autism spectrum disorders, making PCDHGB4 relevant for exploring disease mechanisms. Additionally, it aids in drug discovery for neurodevelopmental conditions and structural studies to map interaction interfaces. Research on PCDHGB4 continues to uncover its role in establishing neural networks and maintaining brain homeostasis.
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