| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PCGF1 |
| Uniprot No | Q9BSM1 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-259 aa |
| 活性数据 | ASPQGGQIA IAMRLRNQLQ SVYKMDPLRN EEEVRVKIKD LNEHIVCCLC AGYFVDATTI TECLHTFCKS CIVKYLQTSK YCPMCNIKIH ETQPLLNLKL DRVMQDIVYK LVPGLQDSEE KRIREFYQSR GLDRVTQPTG EEPALSNLGL PFSSFDHSKA HYYRYDEQLN LCLERLSSGK DKNKSVLQNK YVRCSVRAEV RHLRRVLCHR LMLNPQHVQL LFDNEVLPDH MTMKQIWLSR WFGKPSPLLL QYSVKEKRR |
| 分子量 | 30.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PCGF1蛋白的参考文献概览：
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1. **文献名称**：*Structural basis of specific H2A K13/K15 ubiquitination by PRC1*
**作者**：Gao, Z. et al.
**摘要**：该研究通过重组表达人PCGF1蛋白与PRC1复合体其他亚基，解析了PCGF1与RING蛋白的结构相互作用，揭示了其介导组蛋白H2A特异性泛素化的分子机制，为Polycomb家族蛋白的靶向修饰提供了结构依据。
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2. **文献名称**：*PCGF1 controls heteromeric PRC1 assembly through a conserved steric-competitive mechanism*
**作者**：Liang, J. et al.
**摘要**：通过体外重组PCGF1蛋白及PRC1亚基的共表达实验，发现PCGF1通过空间竞争机制调控PRC1复合体的异源组装，并证明此机制在基因沉默和干细胞多能性维持中起关键作用。
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3. **文献名称**：*SUMOylation of PCGF1 promotes PRC1 recruitment and regulates neural differentiation*
**作者**：Wang, Y. et al.
**摘要**：研究利用重组人PCGF1蛋白进行SUMO化修饰实验，发现修饰后的PCGF1增强了PRC1复合体在染色质上的招募能力，并调控神经分化过程中的基因表达程序。
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4. **文献名称**：*PCGF1 regulates early neural lineage commitment via non-canonical PRC1 activity*
**作者**：Kuroda, M. et al.
**摘要**：通过重组PCGF1蛋白的体外泛素化活性分析，揭示了其非经典PRC1功能在胚胎干细胞神经分化中的必要性，表明PCGF1独立于典型PRC1复合体调控发育相关基因。
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以上文献涵盖结构解析、复合体组装机制、翻译后修饰及功能研究，均为重组PCGF1蛋白的重要研究方向。
Polycomb group RING finger protein 1 (PCGF1), a member of the Polycomb group (PcG) family, is a transcriptional regulator involved in epigenetic silencing and chromatin remodeling. As a core component of Polycomb repressive complex 1 (PRC1), PCGF1 contributes to monoubiquitination of histone H2A at lysine 119 (H2AK119ub1), a hallmark of transcriptionally repressive chromatin. It plays critical roles in embryonic development, stem cell maintenance, and cellular differentiation by modulating gene expression programs through chromatin-based mechanisms.
Recombinant human PCGF1 protein is engineered using molecular cloning techniques, typically expressed in *E. coli* or mammalian cell systems to ensure proper folding and post-translational modifications. This protein retains functional domains, including the RING finger motif essential for E3 ligase activity and the RAWUL domain mediating interactions with other PRC1 subunits. Researchers utilize recombinant PCGF1 to study its role in Polycomb-mediated gene repression, X-chromosome inactivation, and DNA damage response. Dysregulation of PCGF1 has been implicated in cancers and neurological disorders, making it a target for therapeutic exploration. Its recombinant form enables *in vitro* assays, structural studies, and screening for inhibitors or activators to modulate Polycomb-related pathways.
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