| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PI4KII |
| Uniprot No | Q8TCG2 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-481 aa |
| 活性数据 | MEDPSEPDRL ASADGGSPEE EEDGEREPLL PRIAWAHPRR GAPGSAVRLL DAAGEEGEAG DEELPLPPGD VGVSRSSSAE LDRSRPAVSV TIGTSEMNAF LDDPEFADIM LRAEQAIEVG IFPERISQGS SGSYFVKDPK RKIIGVFKPK SEEPYGQLNP KWTKYVHKVC CPCCFGRGCL IPNQGYLSEA GAYLVDNKLH LSIVPKTKVV WLVSETFNYN AIDRAKSRGK KYALEKVPKV GRKFHRIGLP PKIGSFQLFV EGYKEAEYWL RKFEADPLPE NIRKQFQSQF ERLVILDYII RNTDRGNDNW LVRYEKQKCE KEIDHKESKW IDDEEFLIKI AAIDNGLAFP FKHPDEWRAY PFHWAWLPQA KVPFSEEIRN LILPYISDMN FVQDLCEDLY ELFKTDKGFD KATFESQMSV MRGQILNLTQ ALRDGKSPFQ LVQIPCVIVE RSQGGSQGRI VHLSNSFTQT VNCRKPFFSS W |
| 分子量 | 54.7 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PI4KII(Phosphatidylinositol 4-kinase type II)蛋白的3-4篇参考文献示例及简要摘要：
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1. **文献名称**：
**"Functional characterization of recombinant human PI4KIIα in membrane trafficking"**
**作者**：Balla T. et al.
**摘要**：
本研究利用杆状病毒系统表达重组人PI4KIIα蛋白，揭示了其在细胞内膜运输中的作用，证实其通过调控磷脂酰肌醇4-磷酸(PI4P)水平影响高尔基体-质膜囊泡运输过程。
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2. **文献名称**：
**"Structural insights into human PI4KIIβ regulation by small molecules"**
**作者**：Burke J.E. et al.
**摘要**：
通过X射线晶体学解析了重组人PI4KIIβ蛋白与抑制剂结合的复合物结构，阐明了其催化机制及药物靶向潜力，为开发针对脂代谢疾病的治疗策略提供依据。
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3. **文献名称**：
**"Purification and enzymatic activity analysis of recombinant PI4KIIγ from mammalian cells"**
**作者**：Wang Y. et al.
**摘要**：
描述了一种从哺乳动物细胞中高效纯化重组人PI4KIIγ的方法，并验证其体外激酶活性，强调该蛋白在神经细胞PI4P合成中的关键作用。
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4. **文献名称**：
**"PI4KIIα knockdown and recombinant protein rescue experiments reveal its role in autophagy"**
**作者**：Sridhar S. et al.
**摘要**：
通过基因敲除及重组蛋白回补实验，证明PI4KIIα通过调节自噬体形成阶段的PI4P水平，影响细胞自噬过程，提示其在神经退行性疾病中的潜在病理机制。
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(注：以上文献为示例性内容，实际引用请以真实发表的论文为准。)
Phosphatidylinositol 4-kinase type II (PI4KII) is a critical enzyme involved in lipid signaling and membrane trafficking in eukaryotic cells. It catalyzes the phosphorylation of phosphatidylinositol (PI) to generate phosphatidylinositol 4-phosphate (PI4P), a lipid second messenger essential for regulating vesicle formation, organelle identity, and intracellular signaling pathways. Human PI4KII exists in two isoforms, PI4KIIα and PI4KIIβ, which exhibit distinct subcellular localizations and functional roles. PI4KIIα is primarily associated with endosomal and trans-Golgi networks, while PI4KIIβ is enriched in synaptic vesicles and implicated in neuronal functions.
Recombinant human PI4KII protein is produced using heterologous expression systems (e.g., E. coli, insect, or mammalian cells) to enable structural and functional studies. This engineered protein retains kinase activity and is widely used to investigate mechanisms of PI4P production, lipid-protein interactions, and the enzyme's role in diseases. Dysregulation of PI4KII has been linked to neurological disorders, cancer progression, and viral pathogenesis, as certain pathogens exploit PI4P-rich membranes for replication. Inhibitors targeting PI4KII are also explored for therapeutic development, particularly against cancers and RNA viruses like hepatitis C. Research on recombinant PI4KII enhances understanding of cellular lipid dynamics and provides tools for drug discovery.
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