| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIGK |
| Uniprot No | Q92643 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 268-366 aa |
| 活性数据 | MNDLFQVCPKSLCVSTPGHRTDLFQRDPKNVLITDFFGSVRKVEITTETIKLQQDSEIMESSYKEDQMDEKLMEPLKYAEQLPVAQIIHQKPKLKDWHP |
| 分子量 | 36.63 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIGK蛋白的3篇示例参考文献(均为虚构示例，仅用于演示格式)：
1. **标题**: "Functional analysis of recombinant human PIGK in GPI-anchor biosynthesis"
**作者**: Tomita, S. et al.
**摘要**: 本研究成功在大肠杆菌中表达并纯化了重组人PIGK蛋白，证实其具有催化GPI锚定蛋白C端脂质修饰的酶活性，为研究GPI通路缺陷相关疾病提供了工具。
2. **标题**: "Crystal structure of human PIGK reveals insights into its catalytic mechanism"
**作者**: Hirota, Y. et al.
**摘要**: 通过昆虫细胞系统表达重组人PIGK蛋白并解析其晶体结构，揭示了其与其他GPI合成酶(如PIGF)的相互作用界面及底物结合位点。
3. **标题**: "PIGK knockdown and recombinant protein rescue experiments in colorectal cancer models"
**作者**: Ikematsu, N. et al.
**摘要**: 利用哺乳细胞表达的重组人PIGK蛋白进行功能回补实验，证明PIGK通过调控膜蛋白定位影响结肠癌细胞迁移和侵袭能力。
注：以上文献为模拟内容，实际研究中请通过PubMed或Web of Science等数据库检索真实发表文献。
**Background of Recombinant Human PIGK Protein**
Recombinant human PIGK (phosphatidylinositol glycan anchor biosynthesis class K) protein is a key enzyme involved in the biosynthesis of glycosylphosphatidylinositol (GPI) anchors, which tether proteins to cell membranes. PIGK, also known as GPI8 in some species, catalyzes the transamidation step during GPI anchor attachment to newly synthesized proteins in the endoplasmic reticulum. This post-translational modification is critical for the membrane localization and function of numerous cell surface proteins, including receptors, adhesion molecules, and immune regulators.
Defects in PIGK or GPI anchor biosynthesis are linked to inherited diseases such as intellectual disability, epilepsy, and paroxysmal nocturnal hemoglobinuria (PNH). Recombinant human PIGK is typically produced using engineered expression systems (e.g., *E. coli* or mammalian cells) to study its structure, enzymatic mechanisms, or interactions with substrates. Its recombinant form enables in vitro assays, drug screening, and investigations into GPI-related pathologies. Recent structural studies using recombinant PIGK have provided insights into its catalytic domain and substrate-binding regions, aiding the development of targeted therapies. Research on this protein enhances understanding of GPI-anchored protein regulation in health and disease.
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