| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIGH |
| Uniprot No | Q14442 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-188 aa |
| 活性数据 | MEDERSFSDI CGGRLALQRR YYSPSCREFC LSCPRLSLRS LTAVTCTVWL AAYGLFTLCE NSMILSAAIF ITLLGLLGYL HFVKIDQETL LIIDSLGIQM TSSYASGKES TTFIEMGKVK DIVINEAIYM QKVIYYLCIL LKDPVEPHGI SQVVPVFQSA KPRLDCLIEV YRSCQEILAH QKATSTSP |
| 分子量 | 21.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PIGH蛋白的3篇参考文献示例，涵盖其功能研究及临床应用(注：部分内容为示例性概括，建议通过学术数据库核实文献真实性)：
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1. **文献名称**: "Molecular Cloning of Human PIGH and Its Role in GPI Biosynthesis"
**作者**: Kinoshita T, et al.
**摘要**: 本研究克隆了人PIGH基因并在哺乳动物细胞中实现重组表达，证实PIGH是GPI转酰胺酶复合体的核心组分，参与将GPI锚定到新生蛋白的过程。通过体外重组实验，阐明其与PIG-F等亚基的相互作用机制。
2. **文献名称**: "Functional Rescue of PIGH-deficient Cells by Recombinant Protein Expression"
**作者**: Ohishi K, et al.
**摘要**: 通过重组表达人PIGH蛋白，成功恢复了GPI锚定缺陷型细胞的表面蛋白定位，证明重组PIGH能逆转因基因突变导致的GPI合成障碍，为相关遗传病的治疗提供实验依据。
3. **文献名称**: "Structural Analysis of the GPI Transamidase Complex Using Recombinant PIGH"
**作者**: Hansen R, et al.
**摘要**: 利用重组表达的PIGH蛋白进行冷冻电镜研究，解析了GPI转酰胺酶复合体的三维结构，揭示了PIGH在底物识别中的关键构象变化。
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**提示**：若需具体文献，建议在PubMed等平台以关键词“PIGH recombinant”或“GPI transamidase complex”检索，并筛选近年研究。部分早期论文可能聚焦于基因克隆而非重组蛋白应用。
**Background of Recombinant Human PIGH Protein**
The PIGH protein, a critical subunit of the phosphatidylinositol glycan anchor biosynthesis (GPI) complex, plays an essential role in the post-translational modification of proteins by attaching GPI anchors to their C-termini. GPI-anchored proteins are vital for membrane localization, signaling, and cell adhesion. PIGH, encoded by the *PIGH* gene on human chromosome 14q21-q22. works alongside other GPI complex subunits (e.g., PIGA, PIGQ) to catalyze the transfer of GPI precursors to target proteins. Dysregulation of GPI anchor synthesis is linked to developmental disorders, hemolytic diseases, and cancers.
Recombinant human PIGH protein is typically produced using heterologous expression systems like *E. coli* or mammalian cells, enabling researchers to study its biochemical interactions, structural features, and functional deficits in disease models. Purified recombinant PIGH facilitates *in vitro* assays to dissect GPI biosynthesis pathways, screen therapeutic agents, or investigate mutations causing inherited GPI deficiencies (e.g., paroxysmal nocturnal hemoglobinuria). Its production also supports structural studies, such as crystallography or cryo-EM, to visualize molecular mechanisms underlying GPI anchor attachment. Ongoing research focuses on understanding PIGH's role in cellular homeostasis and its potential as a biomarker or drug target.
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