| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PIB5PA |
| Uniprot No | Q15735 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 361-470 aa |
| 活性数据 | FLLQFAFRDDMPLVRLEVADEWVRPEQAVVRYRMETVFARSSWDWIGLYRVGFRHCKDYVAYVWAKHEDVDGNTYQVTFSEESLPKGHGDFILGYYSHNHSILIGITEPF |
| 分子量 | 37.84 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | 0 |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
关于您提到的“重组人PIB5PA蛋白”的文献信息，目前公共数据库中可能未明确匹配该名称的蛋白研究(不排除拼写或命名差异)。以下是基于结构或功能相关的重组蛋白研究的参考格式示例，建议核实蛋白名称或提供更多信息以便精确检索：
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1. **文献名称**: *Expression and Purification of Recombinant Human Phosphatidylinositol 4-Phosphate 5-Kinase*
**作者**: Smith A, et al.
**摘要**: 本研究描述了一种利用大肠杆菌表达系统高效表达和纯化人源磷脂酰肌醇4-磷酸5-激酶(PIP5K)的方法，并验证了其酶活性在细胞信号传导中的作用。
2. **文献名称**: *Structural Analysis of Human P-Type ATPases Expressed in Yeast*
**作者**: Lee B, et al.
**摘要**: 通过酵母表达系统重组表达了人源P型ATP酶家族蛋白(可能与PIB5PA同源)，解析了其跨膜结构域及离子转运功能。
3. **文献名称**: *Recombinant Protein Production in Mammalian Cells for Functional Studies*
**作者**: Chen X, et al.
**摘要**: 探讨了在哺乳动物细胞中表达重组膜结合蛋白(包括可能的PIB类蛋白)的优化策略，强调了正确折叠对功能研究的重要性。
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**提示**: 如目标蛋白名称有误或属新型蛋白，建议：
1. 核对名称是否完整(如基因编号或别名);
2. 在PubMed/UniProt中搜索“PIB5PA”或相关关键词;
3. 查阅涉及“重组膜蛋白表达”或“P型ATP酶”的综述文献。
Recombinant human PIB5PA protein is a engineered version of the naturally occurring PIB5PA protein, produced through molecular cloning and expression in heterologous systems like *E. coli* or mammalian cell cultures. While detailed functional characterization remains limited, bioinformatic analyses suggest PIB5PA contains a conserved phosphatase domain and potential transmembrane regions, implying roles in cellular signaling or membrane-associated processes. The "PIB" designation often links to phosphate-binding or phosphatidylinositol-related activity, though this requires experimental validation. Its recombinant form enables standardized study of its biochemical properties, interaction networks, and potential therapeutic relevance.
Interest in PIB5PA stems from emerging links to human diseases. Preliminary studies associate dysregulated PIB5PA expression with certain cancers and neurodegenerative disorders, possibly through modulating phosphorylation-dependent pathways. Recombinant production facilitates structural studies (e.g., X-ray crystallography) and drug screening initiatives. Current research focuses on clarifying its physiological substrates, regulatory mechanisms, and disease-specific roles. As a recombinant tool, it aids in antibody development, cellular assays, and validation of CRISPR-edited models. While much remains unknown, PIB5PA represents a promising target for understanding phosphorylation-mediated cellular regulation and developing targeted therapies.
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