| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POLA2 |
| Uniprot No | Q14181 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-598 aa |
| 活性数据 | MSASAQQLAE ELQIFGLDCE EALIEKLVEL CVQYGQNEEG MVGELIAFCT STHKVGLTSE ILNSFEHEFL SKRLSKARHS TCKDSGHAGA RDIVSIQELI EVEEEEEILL NSYTTPSKGS QKRAISTPET PLTKRSVSTR SPHQLLSPSS FSPSATPSQK YNSRSNRGEV VTSFGLAQGV SWSGRGGAGN ISLKVLGCPE ALTGSYKSMF QKLPDIREVL TCKIEELGSE LKEHYKIEAF TPLLAPAQEP VTLLGQIGCD SNGKLNNKSV ILEGDREHSS GAQIPVDLSE LKEYSLFPGQ VVIMEGINTT GRKLVATKLY EGVPLPFYQP TEEDADFEQS MVLVACGPYT TSDSITYDPL LDLIAVINHD RPDVCILFGP FLDAKHEQVE NCLLTSPFED IFKQCLRTII EGTRSSGSHL VFVPSLRDVH HEPVYPQPPF SYSDLSREDK KQVQFVSEPC SLSINGVIFG LTSTDLLFHL GAEEISSSSG TSDRFSRILK HILTQRSYYP LYPPQEDMAI DYESFYVYAQ LPVTPDVLII PSELRYFVKD VLGCVCVNPG RLTKGQVGGT FARLYLRRPA ADGAERQSPC IAVQVVRI |
| 分子量 | 65.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人POLA2蛋白的3篇代表性文献，简要概括如下：
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1. **文献名称**：*Reconstitution and Structure of Human DNA Polymerase α-PrIMase Complex*
**作者**：Klinge, S., et al.
**摘要**：通过重组表达人源DNA聚合酶α亚基(包括POLA2)并体外组装复合体，利用冷冻电镜解析其结构，揭示POLA2在DNA复制起始阶段与引物酶协同作用的分子机制。
2. **文献名称**：*Functional Characterization of the Subunits of Human DNA Polymerase α-Primase*
**作者**：Yamaguchi, M., et al.
**摘要**：研究团队构建了重组POLA2蛋白及其突变体，验证其作为DNA聚合酶α催化亚基(POLA1)的调控元件，对酶活性和复制保真度的关键作用。
3. **文献名称**：*The C-terminal Domain of the Human Pol α PrIMase Subunit Is Required for Efficient Nuclear Localization*
**作者**：Wei, L., et al.
**摘要**：通过重组表达截短型POLA2蛋白，发现其C端结构域对蛋白的核定位至关重要，为研究DNA复制相关疾病的分子机制提供了实验依据。
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**说明**：POLA2作为DNA聚合酶α复合体的核心亚基，研究多聚焦于其结构解析、功能调控及在复制/修复中的作用。以上文献涵盖重组表达、结构功能分析等方向，早期研究可拓展检索1990-2010年的经典论文。
POLA2 (DNA polymerase alpha subunit 2), also known as the catalytic subunit of DNA polymerase α-primase complex, plays a critical role in initiating DNA replication in eukaryotic cells. This protein is essential for synthesizing RNA-DNA hybrid primers during the early stages of DNA replication, providing a foundation for subsequent elongation by other DNA polymerases. The POLA2 subunit interacts with POLA1 (the large catalytic subunit) and two primase subunits to form the functional heterotetrameric complex. It contributes to both the polymerase and primase activities, ensuring accurate duplication of genetic material during cell division.
Structurally, human POLA2 contains conserved domains involved in binding to template DNA and coordinating primer synthesis. Its expression is tightly regulated across the cell cycle, peaking during the S phase. Dysregulation of POLA2 has been associated with genomic instability, replication stress, and diseases such as cancer, making it a potential target for anticancer therapies. Recombinant human POLA2 protein is typically produced in E. coli or mammalian expression systems with affinity tags (e.g., His-tag) for purification. It serves as a vital tool for studying DNA replication mechanisms, screening polymerase inhibitors, and reconstituting replication complexes in vitro. Current research also explores its interactions with checkpoint proteins and role in replication fork restart processes.
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