| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | POGK |
| Uniprot No | Q9P215 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-609 aa |
| 活性数据 | MESTAYPLNL SLKEEEEEEE IQSRELEDGP ADMQKVRICS EGGWVPALFD EVAIYFSDEE WEVLTEQQKA LYREVMRMNY ETVLSLEFPF PKPDMITRLE GEEESQNSDE WQLQGGTSAE NEESDVKPPD WPNPMNATSQ FPQPQHFDSF GLRLPRDITE LPEWSEGYPF YMAMGFPGYD LSADDIAGKF QFSRGMRRSY DAGFKLMVVE YAESTNNCQA AKQFGVLEKN VRDWRKVKPQ LQNAHAMRRA FRGPKNGRFA LVDQRVAEYV RYMQAKGDPI TREAMQLKAL EIAQEMNIPE KGFKASLGWC RRMMRRYDLS LRHKVPVPQH LPEDLTEKLV TYQRSVLALR RAHDYEVAQM GNADETPICL EVPSRVTVDN QGEKPVLVKT PGREKLKITA MLGVLADGRK LPPYIILRGT YIPPGKFPSG MEIRCHRYGW MTEDLMQDWL EVVWRRRTGA VPKQRGMLIL NGFRGHATDS VKNSMESMNT DMVIIPGGLT SQLQVLDVVV YKPLNDSVRA QYSNWLLAGN LALSPTGNAK KPPLGLFLEW VMVAWNSISS ESIVQGFKKC HISSNLEEED DVLWEIESEL PGGGEPPKDC DTESMAESN |
| 分子量 | 69.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的参考文献为模拟案例，主要展示格式和内容概括方式。目前公开数据库中关于“重组人POGK蛋白”的研究较为有限，建议您进一步确认蛋白名称拼写或扩展搜索关键词(如全称或功能描述)。
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1. **文献名称**: *Expression and Functional Characterization of Recombinant Human POGK Protein in Escherichia coli*
**作者**: Zhang L, Wang Y, et al.
**摘要**: 本研究通过大肠杆菌系统成功表达并纯化重组人POGK蛋白，发现其具有ATP结合活性，并初步验证了其在MAPK信号通路中的潜在调控作用。
2. **文献名称**: *Structural Insights into POGK Kinase Domain via X-ray Crystallography*
**作者**: Thompson R, et al.
**摘要**: 通过X射线晶体学解析重组人POGK蛋白激酶结构域的三维结构，揭示了其底物结合口袋的特征，为靶向抑制剂设计提供理论依据。
3. **文献名称**: *POGK as a Novel Biomarker in Colorectal Cancer: Role of Recombinant Protein in Cellular Assays*
**作者**: Kim S, et al.
**摘要**: 利用重组人POGK蛋白进行体外细胞实验，证明其在结直肠癌细胞中高表达并通过磷酸化STAT3蛋白促进肿瘤增殖。
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**建议**：若需真实文献，可尝试在PubMed或Google Scholar中搜索 **POGK的全称**(如“Protein kinase POGK”或“POGK Homo sapiens”)或相关功能关键词(如“kinase activity”)，或联系领域专家获取最新研究进展。
Recombinant human POGK (Pogo Transposable Element Derived with KRAB Domain) protein is a genetically engineered product designed to study the biological functions of the native POGK protein. POGK, encoded by the POGK gene, belongs to the KRAB-zinc finger protein family, characterized by an N-terminal KRAB (Krüppel-associated box) domain and C-terminal zinc finger motifs. The KRAB domain typically mediates transcriptional repression through interactions with co-repressors, while zinc fingers facilitate DNA binding, suggesting roles in gene regulation and chromatin remodeling. POGK is thought to influence cellular processes such as proliferation, differentiation, and genomic stability, potentially linked to transposable element suppression or epigenetic modulation. Its recombinant form, produced in systems like *E. coli* or mammalian cells, enables in vitro functional studies, antibody development, and disease mechanism exploration. While its exact physiological roles remain under investigation, dysregulation of POGK has been tentatively associated with cancers and developmental disorders. Recombinant POGK serves as a critical tool for elucidating its molecular interactions, regulatory networks, and therapeutic potential in human diseases. Studies often focus on its DNA-binding specificity, KRAB-mediated protein partnerships, and impact on cellular signaling pathways.
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