| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB25 |
| Uniprot No | P57735 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-210 aa |
| 活性数据 | MGNGTEEDYN FVFKVVLIGE SGVGKTNLLS RFTRNEFSHD SRTTIGVEFS TRTVMLGTAA VKAQIWDTAG LERYRAITSA YYRGAVGALL VFDLTKHQTY AVVERWLKEL YDHAEATIVV MLVGNKSDLS QAREVPTEEA RMFAENNGLL FLETSALDST NVELAFETVL KEIFAKVSKQ RQNSIRTNAI TLGSAQAGQE PGPGEKRACC |
| 分子量 | 23.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为3-4篇关于RAB25蛋白研究的代表性文献(依据知识库内容虚构，实际文献请通过PubMed等平台验证)：
1. **"RAB25 regulates integrin β1 recycling in ovarian cancer progression"**
*作者：Cheng et al. (2014)*
**摘要**：揭示了RAB25通过调控整合素β1的膜再循环增强卵巢癌细胞侵袭性及化疗耐药性的机制。
2. **"RAB25 promotes colorectal cancer growth by suppressing autophagy"**
*作者：Wang et al. (2016)*
**摘要**：发现RAB25与自噬关键蛋白(如LC3)互作，抑制结直肠癌细胞自噬并加速肿瘤进展。
3. **"Rab25 activates EGFR signaling to drive breast cancer metastasis"**
*作者：Zhang et al. (2018)*
**摘要**：阐明RAB25通过稳定EGFR膜定位促进乳腺癌转移及干细胞特性，提示其作为治疗靶点的潜力。
4. **"RAB25 as a prognostic biomarker in gastric cancer"**
*作者：Chen et al. (2020)*
**摘要**：基于临床样本分析，发现RAB25高表达与胃癌患者不良预后相关，可能参与EMT过程。
**注**：以上信息为基于研究热点的模拟概括，具体文献请通过PubMed、Web of Science或Google Scholar以“RAB25”、“cancer”、“membrane trafficking”等关键词检索。
Recombinant human RAB25 protein is a small GTPase belonging to the RAS oncogene superfamily, specifically the RAB GTPase subfamily, which plays pivotal roles in regulating intracellular vesicular trafficking and membrane dynamics. RAB25 is characterized by its GTP-binding domain and conserved structural motifs that enable cycling between active (GTP-bound) and inactive (GDP-bound) states, a mechanism critical for its function in cargo sorting, receptor recycling, and endolysosomal system regulation. It localizes to recycling endosomes and influences epithelial cell polarity, secretion, and absorption processes, particularly in tissues like the intestines and kidneys.
Dysregulation of RAB25 has been implicated in various cancers, where it exhibits context-dependent roles—acting as either an oncogene or tumor suppressor. Overexpression of RAB25 is linked to tumor progression, metastasis, and chemoresistance in ovarian, breast, and colorectal cancers, partly through modulating integrin trafficking, growth factor signaling, and autophagy. Conversely, its downregulation is observed in certain malignancies, suggesting tissue-specific functions. Recombinant RAB25 proteins, produced via bacterial or mammalian expression systems, are essential tools for studying these mechanisms in vitro, enabling interaction assays, structural analyses, and drug discovery efforts. Research on RAB25 continues to explore its therapeutic potential as a biomarker or target for precision oncology.
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