| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB15 |
| Uniprot No | P59190 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-212 aa |
| 活性数据 | MAKQYDVLFRLLLIGDSGVGKTCLLCRFTDNEFHSSHISTIGVDFKMKTIEVDGIKVRIQIWDTAGQERYQTITKQYYRRAQGIFLVYDISSERSYQHIMKWVSDVDEYAPEGVQKILIGNKADEEQKRQVGREQGQQLAKEYGMDFYETSACTNLNIKESFTRLTELVLQAHRKELEGLRMRASNELALAELEEEEGKPEGPANSSKTCWC |
| 分子量 | 25.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB15蛋白的3篇示例参考文献(注：文献信息为示例性内容，实际研究中请根据具体需求检索最新文献):
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1. **文献名称**: "Functional characterization of recombinant human RAB15 in endosomal trafficking"
**作者**: Liu Y, et al.
**摘要**: 本研究在大肠杆菌中成功表达并纯化了重组人RAB15蛋白，通过体外GTP酶活性实验证实其具有典型小G蛋白的特性。进一步细胞实验表明，RAB15通过调控早期内体与高尔基体之间的膜运输，影响表皮生长因子受体(EGFR)的回收过程。
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2. **文献名称**: "RAB15 regulates amyloid precursor protein processing through endosomal pathways"
**作者**: Zhang T, et al.
**摘要**: 利用重组人RAB15蛋白进行体外结合实验，发现其与APP降解相关分子BACE1存在相互作用。过表达RAB15显著促进β-淀粉样蛋白生成，提示其在阿尔茨海默病病理中的潜在作用机制。
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3. **文献名称**: "Structural insights into the GTP-bound active state of human RAB15"
**作者**: Watanabe K, et al.
**摘要**: 通过X射线晶体学解析了重组人RAB15蛋白与GTP类似物复合物的高分辨率结构(1.8 Å)，揭示了其Switch I/II结构域的构象变化特征，为设计靶向RAB15的功能调控分子提供了结构基础。
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如需获取真实文献，建议使用以下检索关键词：
- "recombinant human RAB15 expression"
- "RAB15 GTPase function"
- "RAB15 endosomal trafficking"
在PubMed (https://pubmed.ncbi.nlm.nih.gov) 或Web of Science中查询。
Recombinant human RAB15 protein is a genetically engineered form of the RAB15 GTPase, a member of the Ras-associated binding (RAB) family involved in regulating intracellular membrane trafficking. RAB proteins function as molecular switches, cycling between active GTP-bound and inactive GDP-bound states to control vesicle formation, transport, and fusion. RAB15. encoded by the *RAB15* gene, plays roles in endocytic recycling, endosome dynamics, and cell signaling, with emerging links to neuronal development, immune response, and cancer progression. Its dysregulation has been associated with neurodegenerative disorders and tumor metastasis.
Produced using heterologous expression systems (e.g., *E. coli* or mammalian cells), recombinant RAB15 enables studies of its structure, biochemical interactions, and functional mechanisms. Purification methods often incorporate affinity tags (e.g., His-tag) for isolation, followed by characterization via SDS-PAGE, western blotting, or GTPase activity assays. Researchers utilize recombinant RAB15 to investigate its binding partners, subcellular localization, and regulatory pathways, particularly in membrane trafficking networks. Its application spans in vitro reconstitution experiments, drug screening for RAB-targeted therapies, and elucidating disease-related mutations. As a tool, recombinant RAB15 provides insights into cellular logistics and potential therapeutic targets for membrane-trafficking disorders.
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