| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | R3HDML |
| Uniprot No | Q9H3Y0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 57-253 aa |
| 活性数据 | HISV RDMNALLDYH NHIRASVYPP AANMEYMVWD KRLARAAEAW ATQCIWAHGP SQLMRYVGQN LSIHSGQYRS VVDLMKSWSE EKWHYLFPAP RDCNPHCPWR CDGPTCSHYT QMVWASSNRL GCAIHTCSSI SVWGNTWHRA AYLVCNYAIK GNWIGESPYK MGKPCSSCPP SYQGSCNSNM CFKGLKSNKF TWF |
| 分子量 | 28.6 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人R3HDML蛋白的**示例参考文献**(注：因该蛋白研究可能较少，以下内容为模拟虚构，建议通过权威数据库核实)：
1. **文献名称**：*Structural insights into the R3HDML protein and its RNA-binding mechanism*
**作者**：Smith J, et al.
**摘要**：通过X射线晶体学解析了R3HDML蛋白的三维结构，发现其R3H结构域介导RNA结合，可能与转录后调控相关。
2. **文献名称**：*R3HDML knockdown impairs neuronal differentiation in vitro*
**作者**：Chen L, et al.
**摘要**：研究表明R3HDML在神经干细胞分化中高表达，抑制其表达会阻碍突触形成，提示其在神经发育中的潜在作用。
3. **文献名称**：*Association of R3HDML genetic variants with autoimmune disorders*
**作者**：Wang Y, et al.
**摘要**：通过全基因组关联分析(GWAS)发现R3HDML基因多态性与系统性红斑狼疮的发病风险显著相关。
4. **文献名称**：*R3HDML interacts with PIWI proteins and regulates germline development*
**作者**：Kumar R, et al.
**摘要**：发现R3HDML在生殖细胞中与PIWI蛋白家族互作，可能参与piRNA通路调控，影响配子形成。
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**提示**：
- 实际文献需通过PubMed、Google Scholar等平台，以“R3HDML”或“R3H domain-containing protein”为关键词检索。
- 若相关研究稀缺，建议拓展至“R3H domain proteins”或查阅同家族蛋白(如R3HDM1/2)的文献。
Recombinant human R3HDML (R3H domain-containing protein-like) is a genetically engineered protein derived from the human R3HDML gene, which encodes a polypeptide containing the characteristic R3H domain—a conserved sequence motif known for its potential role in RNA binding and nucleic acid metabolism. The R3H domain typically consists of ~70 amino acids, including an arginine-rich region that may facilitate interactions with RNA or single-stranded DNA. While the exact biological function of R3HDML remains under investigation, it is hypothesized to participate in RNA processing, stability, or translational regulation, potentially influencing cellular processes such as proliferation, differentiation, or stress responses. Recombinant expression systems (e.g., E. coli, mammalian cells) enable high-purity production of R3HDML, bypassing challenges associated with native protein isolation. This facilitates structural studies (e.g., crystallography, NMR), functional assays, and exploration of its interactions with nucleic acids or protein partners. Emerging research suggests possible links between R3HDML dysregulation and diseases, including cancer or neurological disorders, positioning it as a candidate for diagnostic or therapeutic targeting. Its recombinant form is also utilized in developing RNA-focused biotechnological tools. Further characterization of its molecular mechanisms and physiological relevance remains critical to elucidating its role in cellular homeostasis and disease pathology.
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