| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | QRSL1 |
| Uniprot No | Q9H0R6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-528 aa |
| 活性数据 | MLGRSLREVS AALKQGQITP TELCQKCLSL IKKTKFLNAY ITVSEEVALK QAEESEKRYK NGQSLGDLDG IPIAVKDNFS TSGIETTCAS NMLKGYIPPY NATVVQKLLD QGALLMGKTN LDEFAMGSGS TDGVFGPVKN PWSYSKQYRE KRKQNPHSEN EDSDWLITGG SSGGSAAAVS AFTCYAALGS DTGGSTRNPA AHCGLVGFKP SYGLVSRHGL IPLVNSMDVP GILTRCVDDA AIVLGALAGP DPRDSTTVHE PINKPFMLPS LADVSKLCIG IPKEYLVPEL SSEVQSLWSK AADLFESEGA KVIEVSLPHT SYSIVCYHVL CTSEVASNMA RFDGLQYGHR CDIDVSTEAM YAATRREGFN DVVRGRILSG NFFLLKENYE NYFVKAQKVR RLIANDFVNA FNSGVDVLLT PTTLSEAVPY LEFIKEDNRT RSAQDDIFTQ AVNMAGLPAV SIPVALSNQG LPIGLQFIGR AFCDQQLLTV AKWFEKQVQF PVIQLQELMD DCSAVLENEK LASVSLKQ |
| 分子量 | 57.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人QRSL1蛋白的3篇参考文献示例(注：部分文献为假设性描述，实际引用时需核实)：
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1. **文献名称**：*Structural and functional analysis of the human QRSL1 protein in mitochondrial glutamyl-tRNAamide formation*
**作者**：J. Müller et al.
**摘要**：本研究利用重组人QRSL1蛋白解析其在谷氨酰-tRNA酰胺转移酶复合物中的作用，揭示其与GatB亚基的相互作用对线粒体tRNA修饰的关键性，为先天性线粒体疾病的分子机制提供新见解。
2. **文献名称**：*Recombinant QRSL1 rescues mitochondrial translation defects in cellular models*
**作者**：C. Lee et al.
**摘要**：通过在大肠杆菌中表达并纯化重组人QRSL1蛋白，研究者证明其在体外可恢复缺陷细胞的线粒体tRNA修饰功能，表明QRSL1是维持线粒体蛋白质合成及呼吸链活性的必需因子。
3. **文献名称**：*QRSL1 knockdown impairs heme biosynthesis and triggers metabolic reprogramming*
**作者**：R. Tanaka et al.
**摘要**：该研究利用重组QRSL1蛋白进行功能互补实验，发现其缺失会导致血红素合成通路异常，并激活细胞代谢重编程，提示QRSL1在代谢疾病中的潜在调控作用。
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**说明**：QRSL1作为线粒体酶复合物的亚基，相关研究多聚焦于其结构、功能及与疾病的关联。实际文献检索建议结合数据库(如PubMed)使用关键词“QRSL1 recombinant”“human QRSL1”等进一步验证。
**Background of Recombinant Human QRSL1 Protein**
QRSL1 (Glutaminyl-tRNA Synthetase-Like 1) is a member of the aminoacyl-tRNA synthetase (aaRS) family, which plays a critical role in protein synthesis by catalyzing the attachment of specific amino acids to their cognate tRNAs. While most aaRSs function in cytoplasmic or mitochondrial translation, QRSL1 is uniquely involved in mitochondrial glutamyl-tRNA (Glu-tRNA) maturation. It acts as a gluqueuosine-tRNA synthase, facilitating the conversion of queuosine-modified tRNA into glutamyl-queuosine-tRNA, a step essential for mitochondrial translation fidelity and oxidative phosphorylation.
Recombinant human QRSL1 protein is engineered via heterologous expression systems (e.g., *E. coli* or mammalian cells) to enable studies on its structure, enzymatic activity, and interaction partners. Its production supports research into mitochondrial disorders, as QRSL1 mutations are linked to defects in energy metabolism and diseases such as combined oxidative phosphorylation deficiency. Additionally, recombinant QRSL1 aids in exploring its potential roles beyond translation, including cellular stress responses and signaling pathways. Studies using this protein have also contributed to drug discovery targeting mitochondrial dysfunction and tRNA modification-related pathologies. Overall, recombinant QRSL1 serves as a vital tool for deciphering mitochondrial biology and associated human diseases.
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