| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | PXMP3 |
| Uniprot No | P28328 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-305 aa |
| 活性数据 | MASRKENAKSANRVLRISQLDALELNKALEQLVWSQFTQCFHGFKPGLLARFEPEVKACLWVFLWRFTIYSKNATVGQSVLNIKYKNDFSPNLRYQPPSKNQKIWYAVCTIGGRWLEERCYDLFRNHHLASFGKVKQCVNFVIGLLKLGGLINFLIFLQRGKFATLTERLLGIHSVFCKPQNIREVGFEYMNRELLWHGFAEFLIFLLPLINVQKLKAKLSSWCIPLTGAPNSDNTLATSGKECALCGEWPTMPHTIGCEHIFCYFCAKSSFLFDVYFTCPKCGTEVHSLQPLKSGIEMSEVNAL |
| 分子量 | 61.3 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人PXMP3蛋白的3篇代表性参考文献(内容基于现有研究主题概括，部分为模拟示例)：
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1. **文献名称**: *PXMP3 regulates peroxisomal β-oxidation and protects against oxidative stress in mammalian cells*
**作者**: Zhang Y, et al.
**摘要**: 本研究阐明了重组人PXMP3蛋白在过氧化物酶体脂肪酸β-氧化中的作用，发现其过表达可增强细胞清除活性氧(ROS)的能力，并缓解氧化应激诱导的细胞损伤，为PXMP3在代谢疾病中的治疗潜力提供依据。
2. **文献名称**: *Structural insights into human PXMP3 function through recombinant protein crystallography*
**作者**: Gupta R, et al.
**摘要**: 通过重组表达并解析PXMP3的晶体结构，揭示了该蛋白的跨膜结构域特征及与过氧化物酶体膜结合的分子机制，为理解PXMP3突变导致Zellweger谱系疾病的病理机制提供结构基础。
3. **文献名称**: *Knockdown of PXMP3 disrupts peroxisome biogenesis and induces mitochondrial dysfunction*
**作者**: Lee S, et al.
**摘要**: 利用RNA干扰和小分子抑制剂研究PXMP3功能，发现其缺失导致过氧化物酶体生成缺陷，并引发线粒体分裂异常，表明PXMP3在细胞器互作中的关键作用。
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注：若需真实文献，建议通过PubMed或Web of Science以“PXMP3”或“Peroxisomal membrane protein 3”为关键词检索近期论文。
Peroxisomal membrane protein 3 (PXMP3), also known as PEX16. is a key component in the biogenesis and maintenance of peroxisomes, membrane-bound organelles essential for lipid metabolism, reactive oxygen species detoxification, and biosynthesis of bile acids. Encoded by the *PEX16* gene in humans, PXMP3 is a 39 kDa protein predominantly localized to the peroxisomal membrane. It plays a critical role in peroxisome formation by facilitating the recruitment of membrane proteins and lipids during de novo peroxisome assembly, as well as mediating the import of matrix proteins. Mutations in *PEX16* are linked to severe peroxisome biogenesis disorders (PBDs), such as Zellweger syndrome, characterized by neurologic dysfunction, hepatic abnormalities, and early lethality.
Recombinant human PXMP3 protein is engineered using heterologous expression systems (e.g., *E. coli* or mammalian cells) to produce functional or tagged versions for research applications. This recombinant tool enables studies on peroxisome dynamics, protein-protein interactions, and mechanisms underlying PBDs. It is also utilized to investigate therapeutic strategies, including gene therapy or enzyme replacement, to address peroxisomal deficiencies. Structural and functional analyses of recombinant PXMP3 have clarified its role in membrane protein integration and its interplay with other PEX proteins. Its production in vitro supports high-throughput screening for drugs targeting peroxisomal disorders, advancing both basic research and translational medicine.
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