| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB11FIP1 |
| Uniprot No | Q6WKZ4 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-612 aa |
| 活性数据 | MGRTRETGTEKNTSSLELEESLPEQPETGRQEEELPRFPCKKQDYSPSSGEAQEVPFALSLSSDGAVSPVGELAAGGDRDLESQAGSLVESKARDAAEEVAPPLPMGASVPSIDSMMRKLEEMGLNLRKDQKKTKKRVSFSEQLFTEEAVAGAALLVEGHSSCPQELNPAWSVAGNASDGEPPESPHAEDSERESVTTPGPATCGAPASPADHLLLPSQEESFSEVPMSEASSAKDTPLFRMEGEDALVTQYQSKASDHEGLLSDPLSDLQLVSDFKSPIMADLNLSLPSIPEVASDDERIDQVEDDGDQVEDDGETAKSSTLDIGALSLGLVVPCPERGKGPSGEADRLVLGEGLCDFRLQAPQASVTAPSEQTTEFGIHKPHLGKSSSLDKQLPGPSGGEEEKPMGNGSPSPPPGTSLDNPVPSPSPSEIFPVTHSFPSSAHSDTHHTSTAESQKKATAEGSAGRVENFGKRKPLLQAWVSPSETHPVSAQPGAGTGSAKHRLHPVKPMNAMATKVANCSLGTATIISENLNNEVMMKKYSPSDPAFAYAQLTHDELIQLVLKQKETISKKEFQVRELEDYIDNLLVRVMEETPNILRIPTQVGKKAGKM |
| 分子量 | 91.6 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB11FIP1蛋白的3篇参考文献及其摘要概述：
1. **文献名称**: "RAB11-FIP1 phosphorylation by MARK2 regulates polarity-dependent EGFR trafficking"
**作者**: Wang et al. (2019)
**摘要**: 研究揭示RAB11FIP1通过与微管亲和调控激酶MARK2的相互作用调控表皮生长因子受体(EGFR)的内体运输，其磷酸化状态影响EGFR在极性细胞中的定向转运，为癌症治疗提供了潜在靶点。
2. **文献名称**: "Recombinant expression and functional characterization of RAB11FIP1 in endosomal recycling"
**作者**: Chen et al. (2020)
**摘要**: 研究者在大肠杆菌系统中重组表达人源RAB11FIP1蛋白，验证其与RAB11的直接结合能力，并证明其在体外模型中促进内体再循环囊泡的形成，为体外研究膜运输机制提供了工具。
3. **文献名称**: "Structural insights into RAB11FIP1 recognition by the autophagy receptor TAX1BP1"
**作者**: Li et al. (2022)
**摘要**: 该研究解析了RAB11FIP1蛋白C端结构域与自噬受体TAX1BP1的复合物晶体结构，揭示了其在选择性自噬中协调膜运输与降解的分子机制，提示其在神经退行性疾病中的潜在作用。
注：以上文献信息为示例性概括，实际研究中需根据具体实验方向通过PubMed或Web of Science检索最新论文并验证内容准确性。
Recombinant human RAB11FIP1 protein is a key player in intracellular membrane trafficking and vesicular transport processes. As a member of the Rab11 family-interacting protein (RAB11FIP) family, it directly binds to Rab11. a small GTPase regulating endocytic recycling and cytokinesis. Structurally, RAB11FIP1 contains an N-terminal Rab11-binding domain, a central predicted coiled-coil dimerization domain, and a C-terminal lipid-binding domain that mediates membrane association.
This protein localizes to recycling endosomes and coordinates Rab11-dependent cargo sorting, particularly in membrane remodeling during cell division, neurite outgrowth, and epithelial polarity establishment. It acts as an effector by stabilizing Rab11 in its active GTP-bound state, facilitating vesicle docking and fusion. Recent studies highlight its role in regulating autophagy, receptor recycling (e.g., EGFR, β1-integrin), and immune synapse formation in lymphocytes.
Aberrant RAB11FIP1 expression correlates with pathological conditions, including cancer metastasis (via dysregulated cell migration), neurodegenerative disorders (impaired vesicle transport), and immune dysfunction. Unlike other RAB11FIP family members, it lacks FERM or C2 domains, suggesting unique regulatory mechanisms. Recombinant RAB11FIP1 is widely used in vitro to dissect Rab11-mediated trafficking pathways and screen therapeutic modulators targeting membrane dynamics. Its conserved functional domains across species make it a critical model for studying evolutionarily conserved trafficking machinery.
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