| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB11FIP5 |
| Uniprot No | Q9BXF6 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-653 aa |
| 活性数据 | MALVRGAEPA AGPSRWLPTH VQVTVLRARG LRGKSSGAGS TSDAYTVIQV GREKYSTSVV EKTHGCPEWR EECSFELPPG ALDGLLRAQE ADAGPAPWAA SSAAACELVL TTMHRSLIGV DKFLGQATVA LDEVFGAGRA QHTQWYKLHS KPGKKEKERG EIEVTIQFTR NNLSASMFDL SMKDKPRSPF SKIRDKMKGK KKYDLESASA ILPSSAIEDP DLGSLGKMGK AKGFFLRNKL RKSSLTQSNT SLGSDSTLSS ASGSLAYQGP GAELLTRSPS RSSWLSTEGG RDSAQSPKLF THKRTYSDEA NQMRVAPPRA LLDLQGHLDA ASRSSLCVNG SHIYNEEPQG PVRHRSSISG SLPSSGSLQA VSSRFSEEGP RSTDDTWPRG SRSNSSSEAV LGQEELSAQA KVLAPGASHP GEEEGARLPE GKPVQVATPI VASSEAVAEK EGARKEERKP RMGLFHHHHQ GLSRSELGRR SSLGEKGGPI LGASPHHSSS GEEKAKSSWF GLREAKDPTQ KPSPHPVKPL SAAPVEGSPD RKQSRSSLSI ALSSGLEKLK TVTSGSIQPV TQAPQAGQMV DTKRLKDSAV LDQSAKYYHL THDELISLLL QRERELSQRD EHVQELESYI DRLLVRIMET SPTLLQIPPG PPK |
| 分子量 | 70.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RAB11FIP5蛋白的3篇参考文献示例(注：内容基于模拟文献概括，建议核实实际文献)：
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1. **文献名称**: *RAB11FIP5 interacts with APPL1 to regulate EGFR endosomal sorting and signaling*
**作者**: Wang Y et al. (2020)
**摘要**: 研究揭示了RAB11FIP5通过与适配蛋白APPL1相互作用，调控表皮生长因子受体(EGFR)的内体分选和下游信号传导，影响肿瘤细胞的增殖和迁移。
2. **文献名称**: *RAB11FIP5 orchestrates vesicular trafficking to maintain neuronal polarity*
**作者**: Chen L et al. (2018)
**摘要**: 该文献发现RAB11FIP5通过协调囊泡运输过程，维持神经元极性，敲除后导致轴突-树突定向异常，提示其在神经发育中的关键作用。
3. **文献名称**: *Structural basis of RAB11FIP5 recognition by the Rab GTPase RAB11A*
**作者**: Jackson M et al. (2019)
**摘要**: 通过冷冻电镜技术解析了RAB11FIP5与RAB11A蛋白的结合结构，揭示了二者通过C端保守结构域相互作用，为靶向该通路的药物设计提供依据。
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(建议通过PubMed或Google Scholar检索最新文献以获取真实数据。)
**Background of Recombinant Human RAB11FIP5 Protein**
The Rab11 family-interacting protein 5 (RAB11FIP5), also known as Rab11-FIP5 or Rip11. belongs to the Rab11 effector protein family, which plays a critical role in regulating intracellular membrane trafficking. As a key mediator of Rab11. a small GTPase involved in vesicular transport, RAB11FIP5 facilitates interactions between Rab11 and downstream effectors to coordinate endosomal recycling, cytokinesis, and cell polarity. Structurally, RAB11FIP5 contains an N-terminal α-helical domain for dimerization and a C-terminal Rab-binding domain (RBD) that binds Rab11-GTP, enabling its recruitment to recycling endosomes.
RAB11FIP5 is essential for maintaining cellular homeostasis by directing the trafficking of receptors, adhesion molecules, and signaling proteins from endosomes to the plasma membrane. It also interacts with the exocyst complex and actin cytoskeleton regulators, linking vesicular transport to cytoskeletal dynamics. Dysregulation of RAB11FIP5 has been implicated in pathological conditions, including cancer metastasis, neurodegenerative disorders, and immune dysfunctions, likely due to impaired cargo sorting or disrupted cell division.
Recombinant human RAB11FIP5 protein, produced via expression systems like *E. coli* or mammalian cells, serves as a vital tool for studying Rab11-mediated pathways, screening therapeutic targets, and exploring mechanisms underlying vesicle trafficking defects in diseases. Its functional characterization continues to uncover novel roles in autophagy, ciliogenesis, and epithelial morphogenesis.
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