| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB26 |
| Uniprot No | Q9ULW5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-256 aa |
| 活性数据 | MSRKKTPKSK GASTPAASTL PTANGARPAR SGTALSGPDA PPNGPLQPGR PSLGGGVDFY DVAFKVMLVG DSGVGKTCLL VRFKDGAFLA GTFISTVGID FRNKVLDVDG VKVKLQMWDT AGQERFRSVT HAYYRDAHAL LLLYDVTNKA SFDNIQAWLT EIHEYAQHDV ALMLLGNKVD SAHERVVKRE DGEKLAKEYG LPFMETSAKT GLNVDLAFTA IAKELKQRSM KAPSEPRFRL HDYVKREGRG ASCCRP |
| 分子量 | 27.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB26蛋白的3篇文献参考(示例格式，文献信息及内容为虚构演示用)：
1. **文献名称**: *RAB26 regulates lysosome-mediated secretion via interaction with soluble NSF attachment protein receptors*
**作者**: Chen, Y., et al.
**摘要**: 研究了重组人RAB26蛋白与SNARE复合物的相互作用，发现其通过调控溶酶体与细胞膜的融合促进分泌，利用大肠杆菌表达系统获得活性RAB26用于体外功能验证。
2. **文献名称**: *Recombinant RAB26 protein enhances autophagic flux in HeLa cells*
**作者**: Wang, L., et al.
**摘要**: 通过昆虫细胞表达系统制备重组RAB26蛋白，证实其通过结合自噬相关蛋白ATG5-ATG12复合体增强自噬体成熟，为肿瘤治疗提供潜在靶点。
3. **文献名称**: *Structural analysis of RAB26 GTPase bound to GTP analogs reveals nucleotide-dependent conformational changes*
**作者**: Gupta, M., et al.
**摘要**: 利用重组表达纯化的RAB26蛋白进行X射线晶体学研究，解析了GTP/GDP结合状态下的构象差异，揭示其GTP酶活性调控机制。
(注：以上文献为示例，实际引用需查询真实数据库如PubMed或Google Scholar。)
Recombinant human RAB26 protein is a small GTPase belonging to the Ras-associated binding (RAB) family, which plays critical roles in regulating intracellular vesicle trafficking, membrane fusion, and autophagy. RAB26 is primarily involved in secretory pathways, modulating the transport of vesicles between organelles, particularly in specialized cell types like secretory cells and neurons. Studies suggest its function in autophagosome formation, lysosomal degradation, and exocytosis, with implications in cancer, neurodegenerative diseases, and immune responses. Dysregulation of RAB26 has been linked to impaired cargo delivery, disrupted cellular homeostasis, and pathological conditions.
Produced via recombinant DNA technology, the protein is typically expressed in *E. coli* or mammalian cell systems, followed by purification to ensure high specificity and activity. Recombinant RAB26 enables researchers to study its GTP/GDP binding cycle, interactions with effector molecules (e.g., kinases, motor proteins), and regulatory mechanisms in vitro. It also serves as a tool for identifying therapeutic targets, given its involvement in disease-associated pathways. Despite progress, RAB26's exact mechanistic roles and tissue-specific functions remain under investigation, highlighting the importance of recombinant protein-based studies in elucidating its biological and clinical significance.
×
