| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB7 |
| Uniprot No | P51149 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-207 aa |
| 活性数据 | MTSRKKVLLK VIILGDSGVG KTSLMNQYVN KKFSNQYKAT IGADFLTKEV MVDDRLVTMQ IWDTAGQERF QSLGVAFYRG ADCCVLVFDV TAPNTFKTLD SWRDEFLIQA SPRDPENFPF VVLGNKIDLE NRQVATKRAQ AWCYSKNNIP YFETSAKEAI NVEQAFQTIA RNALKQETEV ELYNEFPEPI KLDKNDRAKA SAESCSC |
| 分子量 | 23.4 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于人RAB7蛋白功能的代表性文献的简要概括(模拟示例)：
1. **《RAB7 regulates autophagy in mammalian cells》**
- 作者：Cecconi F. et al.
- 摘要：研究揭示RAB7通过调控自噬体与溶酶体的融合过程参与自噬通路，RAB7的GTPase活性缺陷会导致自噬泡积累，影响细胞代谢稳态。
2. **《RAB7 coordinates lysosomal trafficking and membrane remodeling》**
- 作者：Lippincott-Schwartz J. et al.
- 摘要：利用活细胞成像技术证明RAB7调控溶酶体膜蛋白分布和酸性环境维持，其重组蛋白的过表达可增强晚期内体向溶酶体的物质转运效率。
3. **《RAB7 mutations in neurodegenerative disorders》**
- 作者：Stenmark H. et al.
- 摘要：分析RAB7基因突变体与神经退行性疾病(如CMT2B)的关联，发现突变体RAB7蛋白会干扰轴突运输中囊泡的锚定与释放过程。
4. **《RAB7-mediated endosome positioning regulates cell migration》**
- 作者：Eapen V.V. et al.
- 摘要：首次报道RAB7通过定向运输整合素受体到细胞前缘的特定区域，促进肿瘤细胞的极性迁移和侵袭能力。
注：以上内容为模拟生成，实际文献需通过PubMed/Google Scholar检索确认。建议使用关键词"RAB7 autophagy"/"RAB7 lysosome"/"RAB7 disease"查找近年高分论文。
**Background of Recombinant Human RAB7 Protein**
RAB7. a member of the RAS-associated binding (RAB) GTPase family, plays a pivotal role in regulating intracellular membrane trafficking. As a small GTPase, it cycles between active GTP-bound and inactive GDP-bound states to control vesicular transport processes, particularly late endosome-to-lysosome trafficking and autophagosome-lysosome fusion. Structurally, RAB7 contains conserved GTP-binding domains and a C-terminal prenylation motif for membrane anchoring.
Dysregulation of RAB7 is linked to diseases such as Charcot-Marie-Tooth type 2B (CMT2B), a peripheral neuropathy caused by mutations affecting endolysosomal function. It is also implicated in cancer progression, neurodegenerative disorders (e.g., Alzheimer’s), and pathogen clearance. These associations highlight its importance in cellular homeostasis, signaling, and pathogen defense.
Recombinant human RAB7 protein, produced via expression systems like *E. coli* or mammalian cells, retains post-translational modifications critical for function. It is widely used *in vitro* to study molecular mechanisms of vesicle trafficking, protein-protein interactions, and lysosomal dysfunction. Additionally, it serves as a tool for drug screening targeting RAB7-related pathways, offering potential therapeutic avenues for diseases tied to endolysosomal dysregulation. Its versatility in research underscores its relevance in both basic and applied biomedical studies.
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