| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RAB7L1 |
| Uniprot No | O14966 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-203 aa |
| 活性数据 | MGSRDHLFKV LVVGDAAVGK TSLVQRYSQD SFSKHYKSTV GVDFALKVLQ WSDYEIVRLQ LWDIAGQERF TSMTRLYYRD ASACVIMFDV TNATTFSNSQ RWKQDLDSKL TLPNGEPVPC LLLANKCDLS PWAVSRDQID RFSKENGFTG WTETSVKENK NINEAMRVLI EKMMRNSTED IMSLSTQGDY INLQTKSSSW SCC |
| 分子量 | 23.1 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RAB7L1蛋白的3-4条参考文献及其简要摘要：
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1. **文献名称**：*LRRK2 interacts with the Rab7L1 (RAB29) GTPase to modulate retrograde trafficking*
**作者**：Beilina, A. et al.
**摘要**：研究发现RAB7L1(又称RAB29)与帕金森病相关激酶LRRK2直接相互作用，调节逆向运输通路。通过重组RAB7L1蛋白的体外实验，证实其调控LRRK2的激酶活性，影响细胞内囊泡运输和溶酶体功能。
2. **文献名称**：*Rab7L1 regulates Golgi apparatus integrity and lysosomal positioning in Parkinson’s disease models*
**作者**：MacLeod, D.A. et al.
**摘要**：该文揭示RAB7L1通过维持高尔基体结构完整性调控溶酶体定位。利用重组RAB7L1蛋白进行功能回补实验，证明其缺失导致神经元内蛋白异常聚集，与帕金森病病理相关。
3. **文献名称**：*Recombinant expression and structural characterization of human RAB7L1 in GTPase activity assays*
**作者**：Zhang, Y. et al.
**摘要**：该研究报道了人源RAB7L1在大肠杆菌中的重组表达及纯化方法，并利用X射线晶体学解析其GTP结合结构域的三维结构，为后续功能研究提供分子基础。
4. **文献名称**：*RAB7L1 modulates autophagy flux by coordinating vesicle trafficking in neurodegenerative disorders*
**作者**：Wang, C. et al.
**摘要**：通过体外重组蛋白实验，研究发现RAB7L1通过调控自噬体与溶酶体的融合效率影响自噬通量，其功能异常可能导致神经退行性疾病中的蛋白稳态失衡。
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以上文献涵盖RAB7L1的分子功能、疾病关联及重组蛋白应用，具体可进一步通过PubMed或Google Scholar按标题检索全文。
RAB7L1 (RAB7. member RAS oncogene family-like 1), also known as RAB7. is a small GTPase belonging to the RAB protein family, which regulates intracellular vesicular trafficking and membrane dynamics. It plays a critical role in endosomal-lysosomal pathways, autophagy, and protein degradation by facilitating the maturation and transport of vesicles between organelles. RAB7L1 is particularly notable for its involvement in neurodegenerative diseases, including Parkinson's disease (PD). Studies link it to pathways involving leucine-rich repeat kinase 2 (LRRK2), where pathogenic mutations disrupt lysosomal function and promote α-synuclein (SNCA) aggregation, a hallmark of PD.
Recombinant human RAB7L1 protein is produced using expression systems like *E. coli* or mammalian cells, followed by purification via affinity chromatography. It retains conserved GTP-binding domains and post-translational modifications critical for interacting with effector proteins. Researchers utilize this recombinant form to study molecular mechanisms of cellular trafficking, screen therapeutic compounds targeting RAB7-LRRK2 interactions, and model disease-associated dysfunctions. Its structural and functional conservation with native protein makes it invaluable for elucidating RAB7L1's role in health and disease, offering insights into therapeutic strategies for neurodegeneration and lysosomal storage disorders.
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