| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RABAC1 |
| Uniprot No | Q9UI14 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-185 aa |
| 活性数据 | MAAQKDQQKDAEAEGLSGTTLLPKLIPSGAGREWLERRRATIRPWSTFVDQQRFSRPRNLGELCQRLVRNVEYYQSNYVFVFLGLILYCVVTSPMLLVALAVFFGACYILYLRTLESKLVLFGREVSPEHQYALAGGISFPFFWLAGAGSAVFWVLGATLVVIGSHAAFHQIEAVDGEELQMEPV |
| 分子量 | 45.98 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RABAC1蛋白的3篇参考文献摘要示例(文献信息为假设性示例，供参考)：
1. **"Interaction of PRA1 with Rab3A GTPase: Recruitment to Endosomes through Regulated Binding"**
*作者：Zhen Yan et al. (2003)*
**摘要**：研究揭示了RABAC1(PRA1)与RAB3A的直接相互作用，调控RAB3A从内体到突触囊泡的循环过程，表明其在神经递质释放中的潜在作用。
2. **"Crystallization and Functional Analysis of Recombinant Human RABAC1 in RAB5-Mediated Endosomal Trafficking"**
*作者：Li Xu et al. (2010)*
**摘要**：报道了重组人RABAC1蛋白在大肠杆菌中的高效表达与纯化，并通过晶体结构分析揭示其结合RAB5的关键结构域，阐明其对内体运输的调控机制。
3. **"Overexpression of RABAC1 Alters EGFR Recycling and Promotes Tumor Cell Invasion in Breast Cancer"**
*作者：Chen et al. (2019)*
**摘要**：发现重组RABAC1在乳腺癌细胞中异常表达会通过干扰EGFR再循环促进侵袭，提示其作为癌症治疗靶点的潜力。
**说明**：以上文献为示例，真实研究请通过学术数据库(如PubMed)检索，可尝试关键词“RABAC1/PRA1”、“recombinant human RABAC1”结合研究领域(如“vesicular transport”或“cancer”)查找具体文献。
Recombinant human RABAC1 (Rab acceptor 1) protein is a genetically engineered form of the RABAC1 protein, which plays a critical role in intracellular membrane trafficking and vesicle transport. RABAC1. also known as PRA1 family protein 3. is encoded by the RABAC1 gene located on chromosome 9p13.3. It functions as a Rab-binding cofactor that regulates the membrane association and activity of Rab GTPases, a family of small GTPases essential for vesicle budding, docking, and fusion. RABAC1 is widely expressed in human tissues and localizes to the Golgi apparatus and endosomal compartments, suggesting its involvement in maintaining organelle dynamics and cargo sorting.
The recombinant protein is typically produced in bacterial or mammalian expression systems to ensure proper folding and post-translational modifications. It retains structural features of the native protein, including transmembrane domains and a C-terminal region critical for Rab interaction. Researchers utilize recombinant RABAC1 to study its role in Rab protein cycling, membrane trafficking pathways, and cellular processes like secretion, autophagy, and receptor recycling. Dysregulation of RABAC1 has been implicated in neurological disorders and cancer progression, making it a potential therapeutic target. Its in vitro applications also extend to functional assays, protein interaction studies, and screening for modulators of vesicular transport pathways.
×
