| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RCP9 |
| Uniprot No | O75575 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-148 aa |
| 活性数据 | MEVKDANSAL LSNYEVFQLL TDLKEQRKES GKNKHSSGQQ NLNTITYETL KYISKTPCRH QSPEIVREFL TALKSHKLTK AEKLQLLNHR PVTAVEIQLM VEESEERLTE EQIEALLHTV TSILPAEPEA EQKKNTNSNV AMDEEDPA |
| 分子量 | 16.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是基于假设的重组人RCP9蛋白的参考文献示例，供参考(请注意，RCP9蛋白的名称可能需要核实，可能存在拼写或编号错误)：
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1. **文献名称**: *"Recombinant Human RCP9 Facilitates DNA Repair via Interaction with Homologous Recombination Complexes"*
**作者**: Smith J, et al.
**摘要**: 研究发现重组人RCP9在体外直接结合RAD51蛋白，增强同源重组效率，暗示其在DNA损伤修复中的潜在作用。
2. **文献名称**: *"Crystal Structure Analysis of RCP9: A Novel Regulatory Protein in MAPK Signaling"*
**作者**: Zhang L, et al.
**摘要**: 通过X射线晶体学解析RCP9结构，揭示其具有SH3-like结构域，可能通过调节MAPK通路影响细胞增殖与分化。
3. **文献名称**: *"RCP9 Enhances Metastatic Potential in Breast Cancer through Rab11-Dependent Vesicular Trafficking"*
**作者**: Tanaka K, et al.
**摘要**: 证实RCP9通过与Rab11效应蛋白互作，促进肿瘤细胞囊泡运输，从而加速乳腺癌转移。
4. **文献名称**: *"Development of a Recombinant RCP9-Based Therapeutic for Neurodegenerative Disorders"*
**作者**: Müller R, et al.
**摘要**: 开发高纯度重组RCP9蛋白，并在阿尔茨海默病模型中显示其通过减少tau蛋白过度磷酸化改善神经退行性病变。
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**注意**：上述文献为模拟示例。"RCP9"可能为作者自行命名或存在拼写错误(如实际应为**RCP/Rab11家族成员**、**CDK9**或类似名称)。建议核实名称或补充更多背景信息以获取准确文献。
Recombinant human RCP (RAB coupling protein), also known as RCP9 or RAB11FIP1. is a multifunctional adaptor protein involved in intracellular membrane trafficking and signal transduction. It belongs to the RAB11-interacting protein (FIP) family, which interacts with RAB GTPases to regulate vesicular transport processes. Structurally, RCP contains an α-helical coiled-coil domain and a conserved RAB-binding domain at its C-terminus, enabling interactions with RAB11 and other RAB proteins.
Biologically, RCP plays critical roles in endosomal sorting, receptor recycling (e.g., EGFR, integrins), and maintaining cell polarity. It facilitates communication between endosomal compartments and the plasma membrane, impacting cell migration, cytokinesis, and autophagy. Dysregulation of RCP has been implicated in cancer progression, particularly in breast and ovarian cancers, where it promotes oncogenic signaling and metastasis through enhanced recycling of pro-invasive receptors.
Recombinant RCP proteins are typically produced using bacterial or mammalian expression systems, enabling in vitro studies of its molecular interactions and structural properties. Current research focuses on elucidating its post-translational modifications, tissue-specific isoforms, and potential therapeutic targeting in membrane trafficking-associated diseases.
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