| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | REC8L1 |
| Uniprot No | O95072 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-547 aa |
| 活性数据 | MFYYPNVLQRHTGCFATIWLAATRGSRLVKREYLRVNVVKTCEEILNYVLVRVQPPQPGLPRPRFSLYLSAQLQIGVIRVYSQQCQYLVEDIQHILERLHRAQLQIRIDMETELPSLLLPNHLAMMETLEDAPDPFFGMMSVDPRLPSPFDIPQIRHLLEAAIPERVEEIPPEVPTEPREPERIPVTVLPPEAITILEAEPIRMLEIEGERELPEVSRRELDLLIAEEEEAILLEIPRLPPPAPAEVEGIGEALGPEELRLTGWEPGALLMEVTPPEELRLPAPPSPERRPPVPPPPRRRRRRRLLFWDKETQISPEKFQEQLQTRAHCWECPMVQPPERTIRGPAELFRTPTLSGWLPPELLGLWTHCAQPPPKALRRELPEEAAAEEERRKIEVPSEIEVPREALEPSFPLMVSLEISLEAAEEEKSRISLIPPEERWAWPEVEAPEAPALPVVPELPEVPMEMPLVLPPELELLSLEAVHRAVALELQANREPDFSSLVSPLSPRRMAARVFYLLLVLSAQQILHVKQEKPYGRLLIQPGPRFH |
| 分子量 | 89.1 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下为示例性质的参考文献，基于REC8L1蛋白可能的生物学功能整理(实际文献需根据具体研究补充)：
1. **"REC8L1 maintains chromosome cohesion during mitosis and suppresses tumorigenesis"**
*作者：Smith J, et al.*
**摘要**：研究揭示REC8L1在有丝分裂中调控姐妹染色单体黏连的稳定性，其表达缺失导致染色体错误分离和基因组不稳定性，并与多种癌症发生相关。
2. **"Aberrant expression of REC8L1 in colorectal cancer correlates with poor prognosis"**
*作者：Li X, et al.*
**摘要**：通过分析结直肠癌组织样本，发现REC8L1在肿瘤中异常高表达，且与患者生存率降低显著相关，提示其可能作为癌症预后标志物。
3. **"Structural analysis of the REC8L1 cohesin complex in meiosis"**
*作者：Yamamoto R, et al.*
**摘要**：利用冷冻电镜解析REC8L1在减数分裂中形成的黏连蛋白复合体结构，阐明其与REC8的功能差异及特异性相互作用机制。
4. **"REC8L1 regulates germ cell development in mice"**
*作者：Chen L, et al.*
**摘要**：小鼠模型研究表明，REC8L1缺失导致生殖细胞减数分裂阻滞和配子形成异常，强调其在生殖细胞发育中的关键作用。
**注**：以上为模拟示例，实际文献需通过学术数据库(如PubMed、Web of Science)检索确认。REC8L1功能可能与染色体动力学、细胞分裂及疾病相关，建议结合具体研究方向筛选文献。
**REC8L1 (REC8 Meiotic Cohesin Component-Like 1)** is a protein encoded by the *REC8L1* gene in humans. It belongs to the cohesin complex family, which plays critical roles in chromosome cohesion, segregation, and DNA repair during cell division. Structurally, REC8L1 shares homology with REC8. a meiosis-specific cohesin essential for sister chromatid cohesion in gametogenesis. However, unlike REC8. REC8L1 is primarily expressed in somatic cells and implicated in mitotic cell cycles, though its exact function remains less defined compared to its meiotic counterpart.
Research suggests REC8L1 participates in maintaining genomic stability by ensuring proper chromatid alignment and preventing premature sister separation. It interacts with other cohesin subunits (e.g., SMC1/3. STAG1/2) and regulatory proteins to orchestrate chromatin organization. Dysregulation of REC8L1 has been linked to chromosomal abnormalities, potentially contributing to developmental disorders and cancer. For instance, altered REC8L1 expression is observed in certain tumors, hinting at its role in oncogenesis or tumor suppression.
Despite progress, REC8L1's mechanisms in somatic cells are still under investigation, with studies focusing on its overlap with meiotic pathways, post-translational modifications, and response to DNA damage. Its dual conservation across meiosis and mitosis highlights evolutionary significance, offering insights into cell division regulation and disease pathogenesis.
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