| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RNF38 |
| Uniprot No | Q9H0F5 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-515 aa |
| 活性数据 | MACKISPGAN SASLPGHPNK VICERVRLQS LFPLLPSDQN TTVQEDAHFK AFFQSEDSPS PKRQRLSHSV FDYTSASPAP SPPMRPWEMT SNRQPPSVRP SQHHFSGERC NTPARNRRSP PVRRQRGRRD RLSRHNSISQ DENYHHLPYA QQQAIEEPRA FHPPNVSPRL LHPAAHPPQQ NAVMVDIHDQ LHQGTVPVSY TVTTVAPHGI PLCTGQHIPA CSTQQVPGCS VVFSGQHLPV CSVPPPMLQA CSVQHLPVPY AAFPPLISSD PFLIHPPHLS PHHPPHLPPP GQFVPFQTQQ SRSPLQRIEN EVELLGEHLP VGGFTYPPSA HPPTLPPSAP LQFLTHDPLH QEVSFGVPYP PFMPRRLTGR SRYRSQQPIP PPPYHPSLLP YVLSMLPVPP AVGPTFSFEL DVEDGEVENY EALLNLAERL GEAKPRGLTK ADIEQLPSYR FNPNNHQSEQ TLCVVCMCDF ESRQLLRVLP CNHEFHAKCV DKWLKANRTC PICRADASEV HRDSE |
| 分子量 | 57.5 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RNF38蛋白的假设性参考文献(非真实文献，基于领域知识推测可能的主题)：
1. **《RNF38 mediates p53 ubiquitination to regulate DNA damage response》**
- 作者：Zhang Y, et al.
- 期刊：Cell Reports
- 摘要：研究揭示了RNF38作为E3泛素连接酶，通过泛素化修饰p53蛋白，调控DNA损伤应答中细胞周期停滞的分子机制。
2. **《RNF38 promotes tumor progression by stabilizing c-Myc in breast cancer》**
- 作者：Liu H, et al.
- 期刊：Nature Communications
- 摘要：报道RNF38通过抑制c-Myc的泛素化降解，促进乳腺癌细胞的增殖和侵袭，成为潜在治疗靶点。
3. **《Structural analysis of RNF38 reveals a novel substrate recognition domain》**
- 作者：Xu T, et al.
- 期刊：Journal of Biological Chemistry
- 摘要：解析了RNF38蛋白的结构，发现了其锌指结构域对特定底物识别的关键作用，拓展了对泛素连接酶底物选择机制的理解。
4. **《RNF38 deficiency exacerbates neurodegeneration via disrupting protein homeostasis》**
- 作者：Wang C, et al.
- 期刊：EMBO Reports
- 摘要：证明RNF38通过降解错误折叠蛋白维持神经元稳态，其缺失加速神经退行性疾病中的病理蛋白聚集。
注：以上内容为虚构示例，实际研究需查询真实文献数据库(如PubMed)。
RNF38 (Ring Finger Protein 38) is a member of the E3 ubiquitin ligase family, characterized by a conserved RING (Really Interesting New Gene) finger domain that facilitates substrate recognition and ubiquitin transfer during protein ubiquitylation. Located predominantly in the nucleus, RNF38 plays a regulatory role in critical cellular processes such as DNA damage response, cell cycle progression, apoptosis, and autophagy. It mediates the attachment of ubiquitin molecules to specific target proteins, marking them for proteasomal degradation or modulating their activity. Notably, RNF38 has been implicated in stabilizing and activating the tumor suppressor p53 by counteracting its negative regulator MDM2. thereby influencing genome integrity and stress responses.
Dysregulation of RNF38 is associated with cancer progression, where its overexpression in certain malignancies may promote oncogenic signaling, while its loss correlates with genomic instability. Additionally, emerging studies suggest its involvement in neurodegenerative disorders, potentially through protein homeostasis pathways. Recombinant human RNF38. produced via bacterial or mammalian expression systems, is widely used in biochemical studies to elucidate substrate specificity, enzymatic mechanisms, and interactions within ubiquitination cascades. Its recombinant form also serves as a tool for high-throughput drug screening aimed at targeting ubiquitin-related pathways in disease contexts. Despite progress, the full spectrum of RNF38’s physiological and pathological roles remains under investigation.
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