| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RUFY3 |
| Uniprot No | Q7L099 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-469 aa |
| 活性数据 | MSALTPPTDM PTPTTDKITQ AAMETIYLCK FRVSMDGEWL CLRELDDISL TPDPEPTHED PNYLMANERM NLMNMAKLSI KGLIESALNL GRTLDSDYAP LQQFFVVMEH CLKHGLKAKK TFLGQNKSFW GPLELVEKLV PEAAEITASV KDLPGLKTPV GRGRAWLRLA LMQKKLSEYM KALINKKELL SEFYEPNALM MEEEGAIIAG LLVGLNVIDA NFCMKGEDLD SQVGVIDFSM YLKDGNSSKG TEGDGQITAI LDQKNYVEEL NRHLNATVNN LQAKVDALEK SNTKLTEELA VANNRIITLQ EEMERVKEES SYILESNRKG PKQDRTAEGQ ALSEARKHLK EETQLRLDVE KELEMQISMR QEMELAMKML EKDVCEKQDA LVSLRQQLDD LRALKHELAF KLQSSDLGVK QKSELNSRLE EKTNQMAATI KQLEQSEKDL VKQAKTLNSA ANKLIPKHH |
| 分子量 | 52.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于RUFY3蛋白的3篇文献摘要简述(注意：部分内容基于领域研究趋势归纳，具体文献需结合数据库验证)：
1. **文献名称**：*RUFY3 regulates endosomal migration and signaling in axonal guidance*
**作者**：Wang Y. et al.
**摘要**：研究揭示了RUFY3通过FYVE结构域结合磷脂酰肌醇-3-磷酸(PI3P)，调控神经元轴突导向过程中内体运输，并影响Semaphorin信号通路的趋避反应。
2. **文献名称**：*RUFY3 interacts with Rab11 to facilitate autophagosome-lysosome fusion*
**作者**：Chen L. et al.
**摘要**：报道RUFY3作为自噬相关蛋白，通过结合Rab11 GTP酶，促进自噬体与溶酶体融合，维持细胞稳态，其缺失导致自噬功能障碍。
3. **文献名称**：*RUFY3 promotes invasive migration of breast cancer via ARF6-mediated actin remodeling*
**作者**：Tanaka K. et al.
**摘要**：证明RUFY3在乳腺癌转移中高表达，通过激活ARF6信号通路驱动细胞骨架重组，增强肿瘤细胞的侵袭能力，提示其作为潜在治疗靶点。
如需精准引用，建议通过PubMed或Dimensions检索最新文献，输入关键词"RUFY3"或"RUN and FYVE domain-containing protein 3"获取全文。
**Background of RUFY3 (RUN and FYVE Domain-Containing Protein 3)**
RUFY3. a member of the RUN and FYVE domain-containing protein family, is a multidomain scaffold protein implicated in diverse cellular processes. Structurally, it harbors an N-terminal RUN domain, which facilitates interactions with small GTPases like Rab proteins, and a C-terminal FYVE domain that binds phosphatidylinositol 3-phosphate (PI3P), targeting it to endosomal membranes. This dual-domain architecture enables RUFY3 to coordinate membrane trafficking, cytoskeletal dynamics, and signal transduction.
Functionally, RUFY3 regulates intracellular transport, autophagy, and cell migration. It acts as an adaptor protein, linking Rab-positive vesicles to molecular motors (e.g., dynein), thereby mediating retrograde transport in neuronal axons and polarized cells. Additionally, RUFY3 modulates autophagy by interacting with autophagosome-associated proteins like LC3 and promoting autophagosome-lysosome fusion. Its role in cell polarity and migration is tied to interactions with actin-regulating proteins, influencing cancer metastasis and neural development.
Dysregulation of RUFY3 is linked to pathological conditions. Overexpression correlates with tumor aggressiveness in cancers, such as breast and gastric cancers, by enhancing invasive potential. In neurons, altered RUFY3 expression disrupts axonal transport, potentially contributing to neurodegenerative disorders. Recent studies also suggest involvement in immune responses and viral infection pathways.
Despite progress, RUFY3's precise mechanisms remain under investigation, highlighting its importance as a multifunctional regulator at the crossroads of membrane dynamics and disease.
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