| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RWDD2A |
| Uniprot No | Q9UIY3 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-292 aa |
| 活性数据 | MSASVKESLQ LQLLEMEMLF SMFPNQGEVK LEDVNALTNI KRYLEGTREA LPPKIEFVIT LQIEEPKVKI DLQVTMPHSY PYVALQLFGR SSELDRHQQL LLNKGLTSYI GTFDPGELCV CAAIQWLQDN SASYFLNRKL VYEPSTQAKP VKNTFLRMWI YSHHIYQQDL RKKILDVGKR LDVTGFCMTG KPGIICVEGF KEHCEEFWHT IRYPNWKHIS CKHAESVETE GNGEDLRLFH SFEELLLEAH GDYGLRNDYH MNLGQFLEFL KKHKSEHVFQ ILFGIESKSS DS |
| 分子量 | 33.8 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RWDD2A蛋白的参考文献示例(注：以下内容为模拟示例，实际文献需通过学术数据库验证)：
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1. **文献名称**: *"Functional Role of RWDD2A in DNA Damage Response via Recombinant Protein Interaction Studies"*
**作者**: Zhang Y. et al.
**摘要**: 研究通过重组RWDD2A蛋白的体外实验，发现其与BRCA1复合物的相互作用，表明RWDD2A在DNA损伤修复通路中可能通过调控蛋白质泛素化发挥作用。
2. **文献名称**: *"Structural Characterization of Recombinant Human RWDD2A Using Cryo-EM"*
**作者**: Lee J. & Kim S.
**摘要**: 利用冷冻电镜技术解析重组RWDD2A蛋白的三维结构，揭示了其RWD结构域中的关键氨基酸位点，为后续功能研究提供结构基础。
3. **文献名称**: *"RWDD2A Modulates Wnt/β-catenin Signaling via Recombinant Protein Overexpression in Colorectal Cancer"*
**作者**: Chen H. et al.
**摘要**: 通过重组RWDD2A的细胞过表达实验，证明其通过抑制β-catenin磷酸化调控Wnt通路，影响结肠癌细胞增殖和迁移。
4. **文献名称**: *"Development of a Recombinant RWDD2A-Based ELISA for Autoantibody Detection in Autoimmune Diseases"*
**作者**: Gupta R. et al.
**摘要**: 研究构建了重组RWDD2A蛋白作为抗原的检测方法，发现其在系统性红斑狼疮患者血清中具有特异性自身抗体反应，提示其作为生物标志物的潜力。
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**建议**：若需真实文献，可通过PubMed或Google Scholar搜索关键词(如“RWDD2A recombinant”或“RWD domain containing 2A expression”)，并筛选涉及重组蛋白表达、结构或功能研究的论文。部分研究可能聚焦于基因调控或疾病关联，但实验方法中可能涵盖重组蛋白应用。
**Background of Recombinant Human RWDD2A Protein**
Recombinant human RWDD2A (RWD Domain Containing 2A) protein is a genetically engineered version of the endogenous protein encoded by the *RWDD2A* gene. This protein belongs to the RWD domain-containing family, characterized by a conserved RWD motif involved in protein-protein interactions, potentially regulating ubiquitination, SUMOylation, or other post-translational modifications. RWDD2A is implicated in diverse cellular processes, including stress response, DNA repair, and apoptosis, though its precise molecular mechanisms remain under investigation.
Studies suggest RWDD2A may interact with key signaling pathways, such as the p53 network, influencing tumor suppression or oncogenesis. Altered expression of RWDD2A has been observed in cancers (e.g., colorectal, ovarian), highlighting its potential as a biomarker or therapeutic target. Its recombinant form is typically produced in *E. coli* or mammalian expression systems, retaining functional epitopes for research applications like antibody development, protein interaction studies, and mechanistic analyses of disease pathways.
Current research focuses on elucidating RWDD2A's role in cellular stress adaptation and its crosstalk with ubiquitin-proteasome or SUMO systems. Further exploration may advance understanding of its contribution to cancer progression, neurodegenerative disorders, or immune regulation, positioning it as a molecule of interest in both basic and translational research.
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