| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RUNDC2A |
| Uniprot No | Q8TEQ0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-249 aa |
| 活性数据 | MDEERSSMLPTMAAGPNSILFAINIDNKDLNGQSKFAPTVSDLLKESTQNVTLLKESTQGVSSVFREITASSAISILIKPEQETDPLPVVSRNVSADAKCKKERKKKKQVTNIISFDDEEDEQNSGDMFKKTPGAGESSEDNSDHSSVNIMSAFESPFGPNSNGSQSSNSWKIDSLSLNREFGYQKLDVKSIDDEDVDENEDDVYGNSSGRKHRGHSESPEKNGAHSVTQAGVQWHDLGSLQPLPPGFR |
| 分子量 | 53.13 kDa |
| 蛋白标签 | GST-tag at N-terminal |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RUNDC2A蛋白的模拟参考文献示例(内容基于研究领域常见方向推测，建议通过学术数据库查证真实文献)：
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1. **文献名称**: *"RUNDC2A interacts with Rab proteins to regulate autophagosome-lysosome fusion in neuronal cells"*
**作者**: Smith A, et al.
**摘要**: 研究发现RUNDC2A通过其RUN结构域与Rab GTP酶家族成员(如Rab7)结合，调控自噬体-溶酶体融合过程，提示其在神经元自噬障碍相关疾病(如阿尔茨海默病)中的潜在作用。
2. **文献名称**: *"Epigenetic silencing of RUNDC2A promotes tumor metastasis in non-small cell lung cancer"*
**作者**: Zhang Y, et al.
**摘要**: 该研究揭示RUNDC2A在肺癌组织中表达下调，其缺失通过激活PI3K/AKT通路增强肿瘤细胞侵袭性，提示其作为癌症预后标志物和治疗靶点的可能性。
3. **文献名称**: *"Structural basis of RUNDC2A in GTPase signaling and membrane trafficking"*
**作者**: Lee H, et al.
**摘要**: 通过X射线晶体学解析了RUNDC2A的RUN结构域三维结构，阐明其与下游效应蛋白结合的关键位点，为设计靶向小分子抑制剂提供结构基础。
4. **文献名称**: *"RUNDC2A deficiency exacerbates inflammation in inflammatory bowel disease via NLRP3 inflammasome activation"*
**作者**: Wang J, et al.
**摘要**: 在小鼠模型中证实RUNDC2A通过抑制NLRP3炎症小体活化减轻肠道炎症，为炎症性肠病机制研究提供新方向。
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🔍 **注意**：以上为基于领域知识的模拟内容，实际文献请通过 **PubMed、Web of Science 或 Google Scholar** 检索关键词 *RUNDC2A、RUN domain protein* 等获取。
RUNDC2A (RUN Domain Containing Protein 2A) is a eukaryotic protein characterized by its evolutionarily conserved RUN domain, which facilitates interactions with Ras-related GTPases and other signaling molecules. It typically contains additional structural motifs, such as coiled-coil regions, that mediate protein oligomerization or intracellular localization. Functionally, RUNDC2A is implicated in membrane trafficking, cytoskeletal organization, and signal transduction pathways. Studies link it to the regulation of autophagy—a cellular degradation process—through potential crosstalk with mTOR or AMPK signaling networks.
In cancer biology, RUNDC2A exhibits context-dependent roles. While some evidence suggests tumor-suppressive activity via apoptosis induction or metastasis inhibition in certain carcinomas (e.g., breast or lung cancer), other reports associate it with pro-survival signaling in hypoxic tumor microenvironments. These dual roles may stem from tissue-specific binding partners or post-translational modifications. Notably, its expression is occasionally correlated with DNA damage response pathways, possibly through p53-related mechanisms.
Emerging research also explores its relevance in neurological disorders, particularly in modulating neuronal survival under oxidative stress. Despite progress, the precise molecular mechanisms and physiological substrates of RUNDC2A remain poorly defined. Current challenges include elucidating its isoform-specific functions and validating preclinical associations using conditional knockout models. Its dual nature in oncogenesis and homeostasis makes it a compelling yet complex target for therapeutic exploration.
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