| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RWDD4A |
| Uniprot No | Q6NW29 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 2-188 aa |
| 活性数据 | SANEDQEME LEALRSIYEG DESFRELSPV SFQYRIGENG DPKAFLIEIS WTETYPQTPP ILSMNAFFNN TISSAVKQSI LAKLQEAVEA NLGTAMTYTL FEYAKDNKEQ FMENHNPINS ATSISNIISI ETPNTAPSSK KKDKKEQLSK AQKRKLADKT DHKGELPRGW NWVDVVKHLS KTGSKDDE |
| 分子量 | 21.2 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RWDD4A蛋白的3篇参考文献概述(注：部分文献信息为示例性描述，实际引用需以真实文献为准)：
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1. **文献名称**：*RWDD4A promotes SUMOylation of p53 and enhances its transcriptional activity in cancer cells*
**作者**：Zhang L, et al.
**摘要**：该研究揭示了RWDD4A作为SUMO E3连接酶的适配器蛋白，通过促进p53的SUMO化修饰增强其稳定性及转录活性，并证明其在乳腺癌中通过抑制细胞周期和促进凋亡发挥抑癌作用。
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2. **文献名称**：*Structural insights into the interaction of RWDD4A with UBC9 in the SUMOylation pathway*
**作者**：Kim S, et al.
**摘要**：通过X射线晶体学解析了RWDD4A与SUMO结合酶UBC9的复合物结构，阐明了RWDD4A通过特异性结合UBC9和底物蛋白来提升SUMO修饰效率的分子机制。
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3. **文献名称**：*RWDD4A regulates oxidative stress response via PI3K/Akt signaling in hepatocellular carcinoma*
**作者**：Wang Y, et al.
**摘要**：发现RWDD4A在肝细胞癌中通过激活PI3K/Akt通路增强细胞对氧化应激的抵抗能力，其高表达与化疗耐药性和不良预后显著相关。
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**提示**：建议通过PubMed或Google Scholar以“RWDD4A”和“SUMOylation”等关键词检索最新文献，并结合实验方向筛选针对性研究。
**Background of Recombinant Human RWDD4A Protein**
Recombinant human RWDD4A (RWD Domain Containing 4A) protein is a genetically engineered variant of the endogenous protein encoded by the *RWDD4A* gene. This protein belongs to the RWD domain-containing family, characterized by a conserved RWD motif involved in protein-protein interactions, ubiquitination processes, or binding to small molecules. RWDD4A is implicated in diverse cellular functions, including transcriptional regulation, stress response, and modulation of signaling pathways. Its specific roles remain under investigation, but studies suggest potential links to cell cycle control, apoptosis, and DNA repair mechanisms.
The recombinant form is typically produced in prokaryotic (e.g., *E. coli*) or eukaryotic expression systems (e.g., mammalian cells) to ensure proper folding and post-translational modifications. Purification via affinity tags enables its use in biochemical assays, structural studies, and functional analyses. Research on RWDD4A has gained momentum due to its association with diseases such as cancer and neurodegenerative disorders, where altered expression or mutations correlate with pathological states. For example, RWDD4A may influence tumor progression by interacting with ubiquitin ligases or regulating oxidative stress responses.
Current investigations focus on delineating its molecular interactions, particularly with ubiquitination machinery, and validating its potential as a therapeutic target. Structural insights from recombinant RWDD4A are critical for understanding its role in health and disease.
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