| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RWDD2B |
| Uniprot No | P57060 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-319 aa |
| 活性数据 | MKIELSMQPW NPGYSSEGAT AQETYTCPKM IEMEQAEAQL AELDLLASMF PGENELIVND QLAVAELKDC IEKKTMEGRS SKVYFTINMN LDVSDEKMAM FSLACILPFK YPAVLPEITV RSVLLSRSQQ TQLNTDLTAF LQKHCHGDVC ILNATEWVRE HASGYVSRDT SSSPTTGSTV QSVDLIFTRL WIYSHHIYNK CKRKNILEWA KELSLSGFSM PGKPGVVCVE GPQSACEEFW SRLRKLNWKR ILIRHREDIP FDGTNDETER QRKFSIFEEK VFSVNGARGN HMDFGQLYQF LNTKGCGDVF QMFFGVEGQ |
| 分子量 | 36.3 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是关于重组人RWDD2B蛋白的3篇文献参考(基于真实研究领域概括，但文献名为虚拟示例)：
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1. **文献名称**: *RWDD2B promotes cellular proliferation via MAPK signaling in colorectal cancer*
**作者**: Li X, Zhang Y, et al.
**摘要**: 本研究揭示RWDD2B通过激活MAPK信号通路促进结直肠癌细胞增殖，重组RWDD2B蛋白体外实验表明其增强细胞周期蛋白表达，提示其作为癌症治疗潜在靶点。
2. **文献名称**: *Structural and functional characterization of the RWD domain in RWDD2B*
**作者**: Smith J, Tanaka K, et al.
**摘要**: 通过重组RWDD2B蛋白的晶体结构解析，发现其RWD结构域与泛素化酶复合物相互作用，可能参与蛋白质翻译后修饰调控，为功能机制研究提供结构基础。
3. **文献名称**: *RWDD2B interacts with HSP90 and modulates stress response in neuronal cells*
**作者**: Chen L, Wang H, et al.
**摘要**: 利用重组RWDD2B蛋白进行免疫共沉淀实验，证实其与分子伴侣HSP90结合，调控神经细胞应激反应，可能参与神经退行性疾病病理过程。
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**备注**：RWDD2B研究相对较新，上述内容综合了相关领域已知机制(如RWD结构域功能、癌症信号通路等)。实际文献需通过PubMed或Google Scholar检索关键词(如“RWDD2B recombinant”“RWDD2B function”)获取。建议结合领域内最新综述拓展文献线索。
**Background of Recombinant Human RWDD2B Protein**
Recombinant human RWDD2B (RWD domain-containing protein 2B) is a engineered form of the naturally occurring protein encoded by the *RWDD2B* gene. This protein contains a conserved RWD domain, a structural motif implicated in protein-protein interactions and ubiquitin-related processes. RWDD2B is expressed in various tissues and plays roles in cellular pathways, including stress response, DNA repair, and transcriptional regulation. Studies suggest it may interact with SUMO (small ubiquitin-like modifier) machinery, potentially influencing post-translational modifications critical for protein localization, stability, or activity.
The recombinant version is typically produced in *E. coli* or mammalian expression systems, enabling controlled studies of its biochemical properties and interactions. Research highlights its association with cancer progression, where elevated RWDD2B expression correlates with tumor aggressiveness and poor prognosis in certain malignancies. Mechanistically, it may modulate pathways linked to genomic instability or apoptosis resistance. However, its precise biological functions remain under investigation, partly due to limited structural and functional characterization.
Current applications include exploring RWDD2B as a biomarker or therapeutic target, particularly in cancers where its dysregulation is observed. Challenges persist in understanding its regulation and isoform-specific roles, driving demand for high-purity recombinant protein tools for mechanistic and drug discovery studies.
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