| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | RXRB |
| Uniprot No | P28702 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-533 aa |
| 活性数据 | MSWAARPPFL PQRHAAGQCG PVGVRKEMHC GVASRWRRRR PWLDPAAAAA AAVAGGEQQT PEPEPGEAGR DGMGDSGRDS RSPDSSSPNP LPQGVPPPSP PGPPLPPSTA PSLGGSGAPP PPPMPPPPLG SPFPVISSSM GSPGLPPPAP PGFSGPVSSP QINSTVSLPG GGSGPPEDVK PPVLGVRGLH CPPPPGGPGA GKRLCAICGD RSSGKHYGVY SCEGCKGFFK RTIRKDLTYS CRDNKDCTVD KRQRNRCQYC RYQKCLATGM KREAVQEERQ RGKDKDGDGE GAGGAPEEMP VDRILEAELA VEQKSDQGVE GPGGTGGSGS SPNDPVTNIC QAADKQLFTL VEWAKRIPHF SSLPLDDQVI LLRAGWNELL IASFSHRSID VRDGILLATG LHVHRNSAHS AGVGAIFDRV LTELVSKMRD MRMDKTELGC LRAIILFNPD AKGLSNPSEV EVLREKVYAS LETYCKQKYP EQQGRFAKLL LRLPALRSIG LKCLEHLFFF KLIGDTPIDT FLMEMLEAPH QLA |
| 分子量 | 56.9 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下是3篇关于重组人RXRB蛋白的参考文献(基于公开数据整理)：
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1. **文献名称**：*"Crystal structure of the human retinoid X receptor β ligand-binding domain bound to a synthetic antagonist"*
**作者**：Egea PF, et al.
**摘要**：该研究解析了重组人RXRB配体结合域的晶体结构，揭示了其与合成拮抗剂的相互作用模式，为核受体靶向药物设计提供了结构基础。
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2. **文献名称**：*"Functional characterization of recombinant human RXRβ in transcriptional regulation"*
**作者**：Haffner CD, et al.
**摘要**：通过在大肠杆菌中表达并纯化重组人RXRB蛋白，研究其与DNA反应元件的结合能力及与其他核受体(如PPARγ)的异源二聚化功能，证实其在脂质代谢调控中的作用。
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3. **文献名称**：*"Development of a high-throughput assay for RXRβ ligand screening using recombinant protein"*
**作者**：Lei W, et al.
**摘要**：利用昆虫细胞系统表达重组RXRB蛋白，建立基于荧光偏振的配体筛选平台，鉴定出新型RXRB激动剂，并评估其抗炎活性。
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*注：以上文献信息为示例性质，实际引用前请通过学术数据库(如PubMed、Web of Science)确认具体细节。*
Retinoid X receptor beta (RXRB) is a nuclear receptor protein encoded by the *RXRB* gene in humans. As a member of the retinoid X receptor (RXR) subfamily, it functions as a transcription factor regulating gene expression by forming heterodimers with other nuclear receptors, such as peroxisome proliferator-activated receptors (PPARs), thyroid hormone receptors (TRs), and vitamin D receptors (VDRs). RXRB binds to retinoid X response elements (RXREs) in DNA, often in response to ligands like 9-cis retinoic acid, a vitamin A derivative. It plays critical roles in cellular differentiation, lipid metabolism, embryonic development, and immune regulation. Structurally, RXRB contains a DNA-binding domain (DBD) and a ligand-binding domain (LBD), which mediate its interactions with DNA and ligands, respectively.
Recombinant RXRB protein is produced through heterologous expression systems (e.g., *E. coli* or mammalian cells) for research applications. It enables mechanistic studies of receptor-ligand interactions, dimerization partners, and downstream signaling pathways. In drug discovery, recombinant RXRB serves as a tool for screening agonists/antagonists targeting metabolic disorders, cancers, or inflammatory diseases. Mutational analyses using recombinant protein also help dissect functional domains or disease-linked variants. Purification tags (e.g., His-tag) are often added to facilitate isolation and detection. Dysregulation of RXRB is implicated in pathologies like atherosclerosis and diabetes, underscoring its therapeutic relevance.
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