| 纯度 | >90%SDS-PAGE. |
| 种属 | Human |
| 靶点 | SAMD1 |
| Uniprot No | Q6SPF0 |
| 内毒素 | < 0.01EU/μg |
| 表达宿主 | E.coli |
| 表达区间 | 1-538 aa |
| 活性数据 | MAGPPALPPP ETAAAATTAA AASSSAASPH YQEWILDTID SLRSRKARPD LERICRMVRR RHGPEPERTR AELEKLIQQR AVLRVSYKGS ISYRNAARVQ PPRRGATPPA PPRAPRGAPA AAAAAAPPPT PAPPPPPAPV AAAAPARAPR AAAAAATAPP SPGPAQPGPR AQRAAPLAAP PPAPAAPPAV APPAGPRRAP PPAVAAREPP LPPPPQPPAP PQQQQPPPPQ PQPPPEGGAV RAGGAARPVS LREVVRYLGG SGGAGGRLTR GRVQGLLEEE AAARGRLERT RLGALALPRG DRPGRAPPAA SARPSRSKRG GEERVLEKEE EEDDDEDEDE EDDVSEGSEV PESDRPAGAQ HHQLNGERGP QSAKERVKEW TPCGPHQGQD EGRGPAPGSG TRQVFSMAAM NKEGGTASVA TGPDSPSPVP LPPGKPALPG ADGTPFGCPP GRKEKPSDPV EWTVMDVVEY FTEAGFPEQA TAFQEQEIDG KSLLLMQRTD VLTGLSIRLG PALKIYEHHI KVLQQGHFED DDPDGFLG |
| 分子量 | 56.0 kDa |
| 蛋白标签 | His tag N-Terminus |
| 缓冲液 | PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300. |
| 稳定性 & 储存条件 | Lyophilized protein should be stored at ≤ -20°C, stable for one year after receipt. Reconstituted protein solution can be stored at 2-8°C for 2-7 days. Aliquots of reconstituted samples are stable at ≤ -20°C for 3 months. |
| 复溶 | Always centrifuge tubes before opening.Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100μg/ml. Dissolve the lyophilized protein in distilled water. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
以下提供的是基于SAMD1蛋白相关研究的模拟参考文献(部分内容为示例性概括，若需准确数据请通过学术数据库验证)：
1. **"SAMD1 Acts as a Transcriptional Repressor in Hematopoietic Stem Cell Maintenance"**
- **作者**: Li et al. (2021)
- **摘要**: 揭示了SAMD1通过结合染色质修饰复合物抑制特定基因表达，维持造血干细胞静息状态的功能，为干细胞调控提供新机制。
2. **"Structural Insights into SAMD1’s Role in Chromatin Remodeling"**
- **作者**: Zhang & Wang (2020)
- **摘要**: 通过冷冻电镜解析SAMD1蛋白结构，提出其通过SAM结构域与组蛋白互作，参与染色质重构的分子模型。
3. **"CRISPR Screening Identifies SAMD1 as a Regulator of Innate Immunity"**
- **作者**: Chen et al. (2022)
- **摘要**: 全基因组CRISPR筛选发现SAMD1通过调控干扰素通路抑制病毒反应，可能成为免疫治疗靶点。
4. **"SAMD1 Epitope Characterization for Vaccine Development"**
- **作者**: Kumar et al. (2019)
- **摘要**: 将重组人SAMD1蛋白作为表位进行免疫原性分析，证明其在抗肿瘤疫苗中的潜在应用价值。
**注**：SAMD1研究尚处早期，文献较少，实际引用需在PubMed等平台核实名称准确性(如是否与SAMHD1混淆)。
Recombinant human sterile alpha motif domain-containing protein 1 (SAMD1) is a less-characterized member of the SAM domain protein family, which shares a conserved ~70-amino acid SAM domain involved in protein-protein interactions and molecular scaffolding. SAMD1 is encoded by the *SAMD1* gene located on chromosome 19q13.12 and is expressed in various tissues, though its biological roles remain unclear. Emerging studies suggest it may function as a transcriptional repressor by interacting with chromatin modifiers (e.g., histone deacetylases) or DNA-binding proteins, potentially regulating cell differentiation, proliferation, or epigenetic silencing. Its SAM domain likely facilitates oligomerization or recruitment of partner proteins to specific genomic loci.
Recombinant SAMD1 is produced using heterologous expression systems (e.g., *E. coli* or mammalian cells) for functional studies. Purified recombinant SAMD1 helps investigate its structure, DNA/RNA-binding properties, and interactions with epigenetic regulators. Recent work highlights its potential link to tumor suppression, metabolic pathways, or immune modulation, though mechanistic details are sparse. Research gaps include defining its physiological targets, post-translational modifications, and tissue-specific roles.
Current applications focus on deciphering SAMD1’s role in chromatin remodeling and its therapeutic relevance in diseases like cancer or inflammatory disorders. Its recombinant form serves as a tool for structural biology, high-throughput screening, or validating protein interaction networks. Further studies are needed to map its functional pathways and clinical potential.
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